b354cde1-8cc6-4b29-a1b2-e21fa3e412e9 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2024-07-22 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Development and molecular barcoding of Terebellidae polychaetes from Rothera Research Station, Antarctic Peninsula, Antarctica 2024-07-22 creation 2024-07-22 revision 2024-07-22 publication 2024-07-22 released 1.0 https://doi.org/10.5285/b354cde1-8cc6-4b29-a1b2-e21fa3e412e9 doi GB/NERC/BAS/PDC/01866 https://data.bas.ac.uk/ Please cite this item as: Frontier, N., Clark, M.S., & Peck, L.S. (2024). Development and molecular barcoding of Terebellidae polychaetes from Rothera Research Station, Antarctic Peninsula, Antarctica (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/b354cde1-8cc6-4b29-a1b2-e21fa3e412e9 This dataset set describes the developmental stages and the molecular barcoding of Terebellidae larvae identified from diver-collected samples on 2021-04-22 in Ryder Bay near Rothera Research Station, Antarctic Peninsula, Antarctica. Photographs were collected at seven stages across the larval development. Development was captured from the trochophore stage where the polychaete larvae were bound in mucus to the free swimming planktonic metatrochophore to the nectochaete stage. Molecular barcoding was performed using the 18S rRNA gene at the British Antarctic Survey. Funding: This study was funded by core funding to UKRI NERC-BAS. No credit. completed Frontier, Nadia British Antarctic Survey Researcher author Clark, Melody S British Antarctic Survey Researcher author Peck, Lloyd S British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Biosphere > Zoology > Invertebrates EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates EARTH SCIENCE > Oceans > Marine Biology theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Terebellidae larval development metatrocophore nectochaete trocophore theme Ryder Bay, Antarctic Peninsula Antarctica place Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions Data supplied under Open Government Licence v3.0 otherRestrictions No restrictions apply. url url crossReference url url crossReference textTable eng utf8 biota -68.21003 -68.21003 -67.59547 -67.59547 2021-04-22 2021-04-22 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor image/jpeg text/csv bytes 25270682 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=b354cde1-8cc6-4b29-a1b2-e21fa3e412e9 WWW:LINK-1.0-http--link Get Data Download data download bytes 25270682 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=b354cde1-8cc6-4b29-a1b2-e21fa3e412e9 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Egg samples were collected on 2021-04-22 from Troval dive site, Ryder Bay, using SCUBA. Specimens were maintained in ambient water temperature during transit from the dive site to the Aquarium. Egg samples were transferred into a through-flow aquarium tank where they remained for seven months. Specimens were checked each week and photographed upon noticeable changes in their development. In the Bonner Laboratory aquarium, a subsample of the egg mass was pipetted from the mucus mass and transferred to a petri dish. A portable dissection microscope (Meiji EMZ-TR) was temporarily installed in the aquarium. Photographs were collected using a simple system of aligning an Olympus TG4 camera in the lens viewer, in the absence of a suitable aquarium microscope with a fitted camera. Samples were quickly returned to the aquarium tank. Molecular barcoding using the 18SrRNA gene was performed to identify the larvae. Briefly DNA was extracted from the larvae using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions. Amplification of a 560bp fragment of the 18S rRNA was performed using the primers NSF4 (CTGGTTGATYCTGCCAGT) and NSR581 (ATTACCGCGGCTGCTGGC) and 2x MyTaq HS Readymix (Meridian Biosciences) according to manufacturer's instructions. PCR cycling conditions were as follows: 95 degrees C for 5 mins, the 35 cycles of 95 degrees C for 20 secs; 60 degrees C for 20 secs; 72 degrees C for 30 secs and a final extension of 72 degrees C for 10 mins. The PCR product was Sanger sequenced by Source Bioscience (UK). Blast sequence similarity searching revealed that it was highly likely that these larvae were an uncharacterised Terebellidae species. In the absence of any associated adults for detailed taxonomy, more detailed identification to species level was not possible. The 18SrRNA sequence is available from NCBI under Accession number PP908343. asNeeded completed