b9a2523e-09ba-43c7-acfa-a894a231b133 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:25:36 UK GEMINI 2.3 WGS 84 Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018 2020-04-02 publication https://catalogue.ceh.ac.uk/id/b9a2523e-09ba-43c7-acfa-a894a231b133 10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133 doi: Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L.M., Di Ponzio, R., Ribeiro, G., Assis, R.L., Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre 10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133 The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset depicts the potential activities of the following enzymes: ß-glucosidase, N-acetyl ß-glucosaminidase and phosphomonoesterase at two different soil depths. Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA). The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship). Full details about this dataset can be found at https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133 Lugli, L.F. National Institute of Amazonian Research, Brazil laynaralugli@gmail.com https://orcid.org/0000-0001-8404-4841 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Quesada, C.A.N. National Institute of Amazonian Research, Brazil quesada.beto@gmail.com author Andersen, K.M. Nanyang Technological University, Singapore kelly.andersen@ntu.edu.sg https://orcid.org/0000-0002-1460-9904 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Mercado, L.M. University of Exeter EMAIL NOT PROVIDED https://orcid.org/0000-0003-4069-0838 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Di Ponzio, R. National Institute of Amazonian Research, Brazil rdiponzio@gmail.com https://orcid.org/0000-0002-7527-3594 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Ribeiro, G. National Institute of Amazonian Research, Brazil EMAIL NOT PROVIDED author Assis, R.L. Natural History Museum, University of Oslo, Norway rafale.assis@gmail.com author Hartley, I.P. University of Exeter I.Hartley@exeter.ac.uk https://orcid.org/0000-0002-9183-6617 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Lugli, L. National Institute of Amazonian Research, Brazil laynaralugli@gmail.com pointOfContact Hartley, I. University of Exeter I.Hartley@exeter.ac.uk pointOfContact NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian soil fertiliser GEMET - Concepts, version 4.1.3 2019-01-28 creation Soil Environmental survey Biological Dynamics of Forest Fragments Project (BDFFP) otherRestrictions no limitations otherRestrictions © Natural Environment Research Council otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L.M., Di Ponzio, R., Ribeiro, G., Assis, R.L., Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133 textTable 1000 English utf8 biota environment 2018-01-01 2018-09-30 -68.5 -31.7 -24.6 5.1 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/b9a2523e-09ba-43c7-acfa-a894a231b133 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/b9a2523e-09ba-43c7-acfa-a894a231b133.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Potential activities of 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1.