b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:22:50 UK GEMINI 2.3 Survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions 2025-06-12 publication https://catalogue.ceh.ac.uk/id/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b 10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b doi: Ormsby, M., Woodford, L., White, H., Fellows, R., Oliver, D., Quilliam, R. (2025). Survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions. NERC EDS Environmental Information Data Centre 10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b This dataset contains information relating to the survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions. The data encompass bacterial strain identity, material type, timepoints (days), bacterial concentration (CFU/ml) and survival percentages. Data were sampled over a 28-day period using culture-based approaches; and the ability of isolates to cause disease following their recovery was determined through the use of a Galleria mellonella model. The ability of isolates to persist on materials, dissociate from materials into water bodies, and recolonise materials was also determined. This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1]. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b Michael Ormsby University of Stirling Michael.Ormsby@glasgow.ac.uk pointOfContact Ormsby, M. University of Stirling michael.ormsby@glasgow.ac.uk https://orcid.org/0000-0002-3991-2336 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Woodford, L. University of Stirling Luke.woodford@stir.ac.uk https://orcid.org/0000-0003-2530-2120 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author White, H. University of Stirling Hl-white@outlook.com author Fellows, R. University of Stirling rosie.c.fellows@outlook.com https://orcid.org/0009-0003-8928-2189 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Oliver, D. University of Stirling david.oliver@stir.ac.uk https://orcid.org/0000-0002-6200-562X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Quilliam, R. University of Stirling Richard.quilliam@stir.ac.uk https://orcid.org/0000-0001-7020-4410 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher University of Stirling EMAIL NOT PROVIDED owner Environmental risk Climate and climate change Water quality Biofilm Human Pathogens Plastic Pollution Plastisphere Public Health otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Ormsby, M., Woodford, L., White, H., Fellows, R., Oliver, D., Quilliam, R. (2025). Survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b English utf8 environment Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Data were generated through laboratory experiments investigating the persistence, virulence, and survival of clinically relevant strains of toxigenic Vibrio cholerae. Bacteria were cultured on materials (Polyethylene [PE], polypropylene [PP] and banana leaf [BL]) and analysed for persistence over time (up to 28 days) and virulence using Galleria mellonella larvae. Materials (PE, PP and BL) were prepared and inoculated under controlled laboratory conditions. V. cholerae strains were inoculated onto materials in nutrient-rich and surface water environments. Sampling was taken at defined time points (7, 14, 21, 28 days). G. mellonella larvae were used to evaluate virulence, and survival was assessed over 72 h. The experiments utilised standard laboratory equipment such as 96-well plates and incubators. G. mellonella larvae were sourced from a local supplier and used within one week of delivery. Instrumentation used for bacterial enumeration, including the plate reader, was calibrated according to standard laboratory protocols. The data underwent rigorous quality control through replication (biological triplicates), statistical analysis (ANOVA), and controlled experimental conditions (e.g., temperature and material consistency). Statistical methods including ANOVA and Tukey’s post-hoc tests were applied to assess treatment effects and survival outcomes.