c29d2ce1-0295-439b-adf1-98ed59f73fae English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:22:29 UK GEMINI 2.3 WGS 84 Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a low glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016) 2018-03-19 publication 2016-11-30 creation https://catalogue.ceh.ac.uk/id/c29d2ce1-0295-439b-adf1-98ed59f73fae 10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae doi: Glanville, H.C., De Sosa, L.L., Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a low glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre 10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a low glucose concentration substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured. All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology. The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability. The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae Glanville, H. Bangor University h.c.glanville@keele.ac.uk pointOfContact Glanville, H.C. Bangor University h.c.glanville@keele.ac.uk https://orcid.org/0000-0002-8192-6678 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author De Sosa, L.L. Bangor University afs411@bangor.ac.uk author Marshall, M.R. Centre for Ecology & Hydrology m.marshall@bangor.ac.uk https://orcid.org/0000-0003-2869-0616 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Cooper, D.M. Centre for Ecology & Hydrology enquiries@ceh.ac.uk https://orcid.org/0000-0001-7578-7918 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Jones, D.L. Bangor University d.jones@bangor.ac.uk author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher notPlanned Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Environmental survey liquid scintillation counter North Wales Conwy catchment T2S Turf2Surf Macronutrient Cycling Programme MCP Soil core otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © Bangor University otherRestrictions © UK Centre for Ecology & Hydrology otherRestrictions If you reuse this data, you should cite: Glanville, H.C., De Sosa, L.L., Marshall, M.R., Cooper, D.M., Jones, D.L. (2018). Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a low glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae textTable 10 English utf8 environment 2016-07-15 2016-09-30 -3.784 -3.781 53.201 53.204 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/c29d2ce1-0295-439b-adf1-98ed59f73fae Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/c29d2ce1-0295-439b-adf1-98ed59f73fae.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University. In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity. To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50 ml polypropylene tubes. To determine the rate of 14CO2 evolution, 50 microlitres (µl) of 14C-glucose labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed after 0.5, 1, 2, 4, 6, 24, 48, 72, 96, 120, 144, 168, 192, 336, 504, 672, 840 and 1008 hours and then weekly up to 6 weeks after initial 14C-labelling for the glucose-C additions. On removal, the NaOH traps were mixed with Optiphase HiSafe 3® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G). Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC