c38663cd-2fdf-436a-af4b-c28da67707ba eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2012-03-07 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Krill genetic profiling from samples collected in the Southern Ocean and South Atlantic during the Discovery 2010 cruise JR177 2012-03-07 creation 2012-03-07 revision 2012-03-07 publication 2012-03-07 released 1 c38663cd-2fdf-436a-af4b-c28da67707ba uuid GB/NERC/BAS/PDC/00646 https://data.bas.ac.uk/ Please cite this item as: None Genetic profiling data relating to studies on Antarctic krill, Euphausia superba, that document the sequence of expression of genes over the moult cycle and the spatial-temporal expression of clock genes. This work was carried out to examine rhythmic behaviour patterns in this species - namely diel vertical migration and the moult cycle - and the functioning of the genes that underlie these behaviours. Circadian entrainment experiments were carried out twice during the Discovery 2010 summer cruise (cruise no JR177) using krill caught in nets at latitudes of 60S and 52S. Krill samples from each net were processed and preserved for subequent analysis using molecular biology technique to isolate canonical clock genes. No credit. completed Rosato, Ezio University of Leicester Researcher author Tarling, Geraint British Antarctic Survey Researcher author Shreeve, Rachael Researcher author Kyriacou, Charalambos University of Leicester Researcher author Gaten, Edward University of Leicester Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Agriculture > Animal Science > Animal Ecology and Behavior EARTH SCIENCE > Biosphere > Animal Taxonomy > Crustaceans EARTH SCIENCE > Oceans > Aquatic Sciences > Fisheries EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates EARTH SCIENCE > Oceans > Aquatic Sciences EARTH SCIENCE > Oceans > Marine Biology theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Sea regions Euphausia animal behaviour circadian clock-genes krill microarray moult cycle phylogenetics theme Near South Orkney Islands Southern Ocean North-West of South Georgia South Atlantic Ocean place Agricultural and aquaculture facilities Habitats and biotopes Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license This dataset is supplied under the Open Government Licence v3.0: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ url url crossReference url url crossReference url url crossReference url url crossReference url url crossReference url url crossReference textTable eng utf8 biota oceans -49.0 -33.0 -61.0 -50.0 2007-12-31 2011-06-30 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor bytes 1 http://www.ncbi.nlm.nih.gov/dbEST/ WWW:LINK-1.0-http--link Get Data Download data download bytes 1 http://www.ncbi.nlm.nih.gov/genbank/ WWW:LINK-1.0-http--link Get Data Download data download bytes 1 http://www.ebi.ac.uk/arrayexpress/ WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: An entrainment tank containing around 250 l of flowing seawater was used for studying the spatial and temporal expression of clock genes. The animals were maintained under a controlled light/environmental regime that mimicked that normally experienced by swarms at the latitude from which they were taken. After 2 days entrainment, a sample of krill (usually 10 animals) was taken at 3-hourly intervals over a period of 48 hours; these were either preserved in RNAlater (a commercially available solution for long term storage of tissues without deterioration of their RNA) or were frozen directly at -80C. During the next 24 hours samples were taken at 6-hourly intervals and fixed in PFA as above. The lights were switched off and on the 2nd, 3rd and 4th day in total darkness, the above sampling regime was repeated. The circadian entrainment experiment was carried out twice, using animals from nets at latitudes of 60S and 52S. A total of 1,090 preserved specimens were returned to the UK for subsequent molecular genetic analysis. A second entrainment tank was used to study the moult cycle genes. Up to 20 animals were removed each day at the same time, measured, moult staged and the heads preserved in RNAlater for subsequent use in the microarray studies to isolate the genes involved in the moult cycle. A total of 590 krill in RNAlater were returned to the UK at -20C for further analysis. The ambient water temperatures for these latitudes were taken from CTD data. Data collection: See the following for details: Seear PJ, Tarling GA, Burns G, Goodall-Copestake WP, Gaten E, Ozkaya O, Rosato E (2010). Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) BMC Genomics, 11: 582. Seear P J, Tarling GA, Teschke M, Meyer B, Thorne MAS , Clark MS, Gaten E, Rosato E (2009) Effects of simulated light regimes on gene expression in Antarctic krill (Euphausia superba Dana) Journal of Experimental Marine Biology and Ecology, 381: 57-64 asNeeded completed