cce747ad-7536-4669-a244-d8bc48693cc7 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:26:59 UK GEMINI 2.3 Multivariate life history phenotype of 56 Daphnia magna clones reared in four different environments (factorial cross of temperature x food) 2022-06-01 publication https://catalogue.ceh.ac.uk/id/cce747ad-7536-4669-a244-d8bc48693cc7 10.5285/cce747ad-7536-4669-a244-d8bc48693cc7 doi: Brunner, F.S., Reynolds, A., Price, S., Atkinson, D.A., Paterson, S., Plaistow, S.J. (2022). Multivariate life history phenotype of 56 Daphnia magna clones reared in four different environments (factorial cross of temperature x food). NERC EDS Environmental Information Data Centre 10.5285/cce747ad-7536-4669-a244-d8bc48693cc7 This dataset contains information about the multivariate plastic life-history responses and thermal tolerance capability of 56 Daphnia magna clones exposed to 4 different environments. The experiment was conducted in a laboratory at the University of Liverpool in 2017. The work was part of a larger mesocosm study that aimed to tease apart how plasticity and genetic diversity influence the evolutionary potential of natural populations. Life history data were measured in common garden experiments. For each clone of the same genotype across all environments, we measured: length at maturity; length at second clutch; age at maturity; age at second clutch; juvenile growth rate ((length at maturity - length as neonate) / age at maturity); adult growth rate ((length at second clutch - length at maturity) / (age at second clutch-age at maturity)); average fecundity (across clutches 1 and 2); and average offspring size (across clutches 1 and 2) The work was supported by the Natural Environment Research Council (Grant NE/K007394/1). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/cce747ad-7536-4669-a244-d8bc48693cc7 Brunner, F.S. University of Liverpool Franziska.Brunner@liverpool.ac.uk https://orcid.org/0000-0002-6571-6945 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Reynolds, A. University of Liverpool alan.reynolds1616@yahoo.com author Price, S. University of Liverpool price@mail.holar.is author Atkinson, D.A. University of Liverpool davida@liverpool.ac.uk https://orcid.org/0000-0002-9956-2454 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Paterson, S. University of Liverpool S.Paterson@liverpool.ac.uk https://orcid.org/0000-0002-1307-2981 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Plaistow, S.J. University of Liverpool S.Plaistow@liverpool.ac.uk https://orcid.org/0000-0002-9003-6271 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Plaistow, S. University of Liverpool S.Plaistow@liverpool.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian University of Liverpool S.Plaistow@liverpool.ac.uk owner Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Daphnia magna Wikidata 2012-10-29 creation Phenotypic plasticity Thermal tolerance CTMax otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Brunner, F.S., Reynolds, A., Price, S., Atkinson, D.A., Paterson, S., Plaistow, S.J. (2022). Multivariate life history phenotype of 56 Daphnia magna clones reared in four different environments (factorial cross of temperature x food). NERC EDS Environmental Information Data Centre https://doi.org/10.5285/cce747ad-7536-4669-a244-d8bc48693cc7 English utf8 biota 2017-01-01 2017-12-31 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/cce747ad-7536-4669-a244-d8bc48693cc7 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/cce747ad-7536-4669-a244-d8bc48693cc7.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 The data were collected by the authors as part of a NERC Highlight grant (Grant NE/N016017/1). 1) Daphnia magna clones used in the experiment were isolated from Brown Moss in Shropshire (52 deg 57' 01'' N 2deg 39' 05'' W) and Lake Ring in Denmark (55 deg 57' 51.83'' N, 9 deg 35' 46.87'' E) and the Camargue in Southern France as hatched resting eggs. 2) The plasticity of each clone was assayed by rearing all clones in common garden studies using 4 different environments (factorial cross of food and temperature). 3) The life-history of the 56 D. magna clones in each environment was assayed by photographing neonates, upon reaching maturity (measured as eggs in the brood pouch) and upon releasing their second clutch, using a Canon EOS 350D digital camera connected to a Leica MZ6 dissecting microscope. The number of neonates each individual produced in their 1st and 2nd clutch were counted and five neonates in each clutch were photographed to obtain an estimate of offspring size. Body size was measured as the distance from the top of the head to the base of the tail-spine using the image analysis software, (ImageJ version 1.45s). After mothers released their second clutch, their thermal tolerance (CTmax) was assayed as described by Geerts et al. (2015) allowing animals an acclimation period of 15 mins at 21 deg C followed by a ramping period of 40 s/deg C from 21 deg C to 50 deg C. All data were checked, verified and processed for analysis. Critical thermal maximum (CTmax) was measured as in (Geerts et al., 2015).