Date (Creation)

2023-12-12

Date (Revision)

2023-12-12

Date (Publication)

2023-12-12

Date (released)

2023-12-12

Edition

2.0

Unique resource identifier

https://doi.org/10.5285/cf3133fb-0bd5-48e5-a89c-d464bd5b90a3

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01807

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Newsham, K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures - VERSION 2.0 (Version 2.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/cf3133fb-0bd5-48e5-a89c-d464bd5b90a3

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Newsham, Kevin		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Fungi

Theme

• Antarctic heatwaves

• beta-glucosidase

• cellulase

• fluctuating temperature

• growth rate

• psychrotrophy

• soil fungi

Place

• Lewis Pass, South Georgia

• The Backslope, Signy Island

• Walton Terraces, Leonie Island

• Mars Oasis, Alexander Island

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Data released under Open Government Licence V3.0:

Use the following citation:

Newsham, K. K. (2023). Growth rates and specific cellulase activities of Antarctic Pseudogymnoascus roseus isolates exposed to constant and fluctuating temperatures. (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/3EF187B8-3471-42AB-BC5D-D7E5E6F5D575

Use constraints

Other restrictions

Other constraints

No restrictions apply.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

doi

Codespace

doi

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2021-09-01

End date

2023-03-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

A total of 10 isolates of P. roseus were obtained from soils at Lewis Pass on South Georgia (two isolates, SG1 and SG2), The Backslope on Signy Island (two isolates, S1 and S2), Walton Terraces on Leonie Island (three isolates, L1-L3) and Mars Oasis on Alexander Island (three isolates, A1-A3). They were maintained on half-strength potato dextrose medium.

Three experiments were conducted. In the first, each isolate was inoculated onto soil extract medium in eight Petri dishes, which were incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony fronts were then marked on dish reverses with a fine blade at × 10-15 magnification (accuracy c. 100 micrometers) and four dishes inoculated with each isolate were transferred to cabinets fluctuating daily from 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius and 2-24 degrees Celsius. The other four dishes inoculated with each isolate were transferred back to the cabinets running at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius. Note that the constant temperatures were the means of the fluctuating temperatures (e.g., 9.0 degrees Celsius was the mean of the temperatures fluctuating from 2-15 degrees Celsius). Colony fronts were marked on dish reverses 24 hours and 48 hours later, and mean growth rate (mm / day) was calculated.

In the second experiment, soil extract medium in eight Petri dishes was again inoculated with each of the 10 isolates, which were again incubated at constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius for one week. Colony radii were then measured twice at right angles to each other at × 10-15 magnification. Four dishes per isolate that had been incubated at each of these temperatures were then placed into cabinets set to fluctuating temperatures of 2-15 degrees Celsius, 2-18 degrees Celsius, 2-21 degrees Celsius or 2-24 degrees Celsius, respectively, and the remaining four dishes per isolate were replaced in the cabinets set to constant temperatures of 9.0 degrees Celsius, 10.7 degrees Celsius, 12.0 degrees Celsius or 13.1 degrees Celsius. Colony radii were measured every 3-5 days over the next 21 days and mean growth rates (mm / day) were calculated by regressing colony radii against time. After 21 d, extracellular beta-glucosidase (cellulase) was extracted from colonies in 10 mM phosphate buffer. Protein concentrations in the buffer were measured using a Micro BCA Protein Assay (Thermofisher). Cellulase activity was measured and expressed as nmol of p-nitrophenol released from 4-nitrophenyl-beta-D-glucopyranoside / min / microgammes protein (Misiak et al. 2021, doi: 10.1111/gcb.15456).

In the third experiment, the 10 isolates were each inoculated onto soil extract medium in four Petri dishes, which were incubated at constant temperatures of 2.0 degrees Celsius, 5.5 degrees Celsius, 8.5 degrees Celsius, 15.0 degrees Celsius, 18.0 degrees Celsius, 21.0 degrees Celsius and 24.0 degrees Celsius for one week. Colony fronts were then marked at × 10-15 magnification on dish reverses as described above. The fronts were marked again after 24 hours and 48 hours and radial extension rate (mm / day), averaged over 48 hours, was calculated.

This dataset was updated to include additional data from the third experiment described above and to update the metadata accordingly.

Data collection:

Micro BCA Protein Assay (Thermofisher)

Data quality:

Data have not been cleaned. Data losses, indicated by MD (missing data) were caused by laboratory errors. Note that the accuracy of growth rate data measured at 12 degrees Celsius and 2-21 degrees Celsius over 0-48 hours was lower than for other growth rate data.

Metadata

File identifier

cf3133fb-0bd5-48e5-a89c-d464bd5b90a3 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2023-12-12

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact