d7b9c848-2bd2-4c33-b017-151116081abf English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2026-01-09T12:11:44 UK GEMINI 2.3 WGS 84 / Pseudo-Mercator Soil biogeochemical data from an incubation experiment testing freeze-thaw and drought effects on alpine soil from Vent, Austria 2025-11-28 publication https://catalogue.ceh.ac.uk/id/d7b9c848-2bd2-4c33-b017-151116081abf 10.5285/d7b9c848-2bd2-4c33-b017-151116081abf doi: Broadbent, A., Pritchard, W., Newbold, L., Giunta, A., Schloter, M., Bahn, M., Griffiths, R., Bardgett, R. (2025). Soil biogeochemical data from an incubation experiment testing freeze-thaw and drought effects on alpine soil from Vent, Austria. NERC EDS Environmental Information Data Centre 10.5285/d7b9c848-2bd2-4c33-b017-151116081abf [This dataset is embargoed until August 30, 2027]. This dataset contains information about soil biogeochemical parameters from a lab manipulation experiment that was part of a study testing freeze-thaw and drought effects on alpine soil microbial communities and biogeochemistry. The soil was collected from Vent, Austria in March 2021. It was then shipped to the Soil and Ecosystem Ecology Lab at the University of Manchester, where the experiment was conducted in April - August 2021. Various soil biogeochemical properties were measured on destructively harvested samples at five experimental timepoints. The soil samples had been subjected to different treatment combinations throughout the experiment (see lineage for details). For details on each parameter, see the supporting documentation. Full details about this dataset can be found at https://doi.org/10.5285/d7b9c848-2bd2-4c33-b017-151116081abf Arthur Broadbent University of Stirling arthur.broadbent@stir.ac.uk pointOfContact Broadbent, A. University of Stirling arthur.broadbent@stir.ac.uk https://orcid.org/0000-0002-8438-7163 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Pritchard, W. University of Manchester william.pritchard@manchester.ac.uk author Newbold, L. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk author Giunta, A. Universität Innsbruck adgiunta@gmail.com author Schloter, M. Helmholtz Zentrum München schloter@helmholtz-muenchen.de author Bahn, M. Universität Innsbruck Michael.Bahn@uibk.ac.at author Griffiths, R. Bangor University robert.griffiths@bangor.ac.uk author Bardgett, R. Lancaster University r.d.bardgett1@lancaster.ac.uk author University of Manchester EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Climate and climate change Soil alpine biogeochemistry climate change otherRestrictions Embargoed otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Broadbent, A., Pritchard, W., Newbold, L., Giunta, A., Schloter, M., Bahn, M., Griffiths, R., Bardgett, R. (2025). Soil biogeochemical data from an incubation experiment testing freeze-thaw and drought effects on alpine soil from Vent, Austria. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/d7b9c848-2bd2-4c33-b017-151116081abf textTable English utf8 environment biota 10.897 10.897 46.863 46.863 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Soil (10-cm depth) was collected in the field (field site near Vent, Austria) using a spade and shipped to the University of Manchester on ice. An incubation experiment was run testing freeze-thaw and drought effects. The experimental treatments included freeze-thaw cycle frequency (0, 1, 2 and 4 cycles of freezing and thawing) followed by drought intensity (9 point gradient of 10-60% Water Holding Capacity (WHC) of soil). Samples were destructively harvested and soil biogeochemical data were measured using a Seal AA3 Segmented Flow Multi-chemistry analyser (Seal Analytical, UK), a 5000A TOC analyser (Shimadzu, Japan), and an Elementar Vario EL elemental analyser (Hanau, Germany) at the Soil and Ecosystem Ecology Lab at the University of Manchester. Specifically, dissolved organic carbon (DTOC) and nitrogen (DON); dissolved NH4+ (DNH4) and NO3- (DNO3); KCl-extracted NH4+ (ENH4) and NO3- (ENO3); Microbial biomass C (MBC), N (MBN), and C:N ratio (MBCN); soil %C (pct_C), %N (pct_N) and C:N ratio (CN_ratio). All instruments were calibrated to cover the full range of sample values. This varied depending on the timepoint, instrument and variable being analysed. Standard practice and protocols within the Soil Ecology and Ecosystem lab at the University of Manchester were followed at all times. Any samples that fell outwith the calibrated range of the instrument were re-analysed with a new calibration to cover the extended range. Appropriate quality control standards were inserted approximately every 20 samples on every run on each instrument to determine the readings were accurate. Three blanks of the extraction matrix (i.e., water, KCl or K2SO4) were measured during every run and subtracted from the samples to account for background concentrations. If errors were detected, the instruments were repaired, and samples were re-run. Raw data were converted into concentrations (per gram of dry soil).