BGS Hosted Metadata
Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016
This dataset contains taxanomic and neuropil volume measurements of wild caught heliconiine butterflies, as well as kenyon cell and synapse counts in the mushroom body calyx of insectory -reared heliconiine butterflies. Wild caught butterflies were sampled from five locations in Central and South America between 2012 and 2016. Locations included Costa Rica, Panama, French Guiana, Ecuador, and Peru. These locations covered a range of habitats from dry to wet forest, at elevations from sea level to 2500m. Interspecific data from wild caught individuals include identification to a species level, sex, and body size, as well as volume measurements of the mushroom body structures, central brain, medulla, and antennal lobe. Measurements of mushroom body calyx volume, and estimates of Kenyon cell and synapse counts are included for six species of insectary-reared adult heliconiine butterflies (Agraulis vanilla, dryas iulia, eueides Isabella, heliconius melpmonene, heliconius Hortense, heliconius hecale). Two species (dryas iulia, heliconius charthonia) were also sampled for microglomeruli counts. This dataset was created as part of a NERC Independent Research Fellowship NE/N014936/1, to analyse the evolutionary expansions in mushroom body size in Heliconius butterflies, compared to their closest relatives. Full details about this dataset can be found at https://doi.org/10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882
Simple
- Date (Publication)
- 2024-11-15
- Citation identifier
- https://catalogue.ceh.ac.uk/id/de62eff7-6ea4-44d7-a015-2eb9b25cf882
- Citation identifier
- doi: / 10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882
- Other citation details
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Montgomery, S., Couto, A. (2024). Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016. NERC EDS Environmental Information Data Centre 10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Bristol Montgomery, S. Point of contact University of Bristol Montgomery, S. Author University of Bristol Couto, A. Author NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher University of Bristol Owner
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GEMET - INSPIRE themes, version 1.0
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Species Distribution
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- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
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If you reuse this data, you should cite: Montgomery, S., Couto, A. (2024). Taxonomic data, neuropil volume measurements, and mushroom body calyx kenyon cell and synapse counts for Heliconiini butterflies, Central and South America, 2012-2016. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/de62eff7-6ea4-44d7-a015-2eb9b25cf882
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Begin date
- 2012-01-01
- End date
- 2016-12-31
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- Unique resource identifier
- WGS 84
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/de62eff7-6ea4-44d7-a015-2eb9b25cf882 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/de62eff7-6ea4-44d7-a015-2eb9b25cf882.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Samples were collected over multiple field seasons across central and south America using a hand-net, fixed in situ and returned to the UK. Fixed samples were then stained with Anti-SYNORF as a primary antibody, cy2 as a secondary antibody, and imaged using confocal microscopy. The resulting images, which highlight boundaries between brain regions, were then segmented using AMIRA software to quantify neuropil volumes. Insectary-reared samples were obtained using pupae from commercial supplies. Freshly eclosed butterflies were aged in a controlled temperature room at 28 degrees Celsius with 80% humidity with a 12-hour day/night cycle. The cage was enriched with a natural food resource, supplemented by artificial feeders. Butterflies were sampled six to ten days after emergence. Kenyon cell counts were estimated using immunohistochemical staining against DAPI, imaged with confocal microscopy. Synapse density estimates were made using higher magnification imaging of Anti-SYNORF stained brains, focusing on the calyx. Total count data was obtained by estimating nuclei density/synapse density within sub-sampled regions of a structure, and then extrapolating across the total volume of the structure.
Metadata
- File identifier
- de62eff7-6ea4-44d7-a015-2eb9b25cf882 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-03-21T09:32:49
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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