e115d087-e3e4-4794-b4e0-18262b8e6400 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2025-10-08 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Benthic, epibenthic and pelagic marine invertebrate specimens collected during PS136 in the Arctic Fram Strait in 2023 and their individual lipid profiles and bulk isotopes 2025-10-08 creation 2025-10-08 revision 2025-10-08 publication 2025-10-08 released 1.0 https://doi.org/10.5285/e115d087-e3e4-4794-b4e0-18262b8e6400 doi GB/NERC/BAS/PDC/02090 https://data.bas.ac.uk/ NE/W004933/1 award Please cite this item as: Linse, K., Ford, D., Blackbird, S.J., Brix, S., Freer, J.J., Jeffreys, R., Schmidt, L.A., & Mayor, D.J. (2025). Benthic, epibenthic and pelagic marine invertebrate specimens collected during PS136 in the Arctic Fram Strait in 2023 and their individual lipid profiles and bulk isotopes (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/e115d087-e3e4-4794-b4e0-18262b8e6400 During the expedition PS136 LTER HAUSGARTEN in 2023 105 samples of macrobenthic marine invertebrates and 3 samples of calanoid copepods were collected by epibenthic sledge and 28 samples of calanoid copepods were collected by Bongo and Multinet and subsequently frozen at -80 degrees Celsius for lipid and bulk stable isotope analyses. Katrin Linse, Saskia Brix, Jannifer Freer and Lydia Anastasia Schmidt collected, photographed and fixed the specimens on board. The macrobenthic invertebrates and calanoid copepods were collected to study elements of the biological lipid pump in the Arctic Ocean. The preparation and lab analyses of the lipid biomarker and stable isotope data were carried out by Rachel Jeffreys and Sabena Jane Blackbird at the University of Liverpool using Gas chromatography-mass spectrometry and an ECS8020 elemental analyser (NC Technologies) coupled to a Delta V isotope ratio mass spectrometer. Statistical analyses and interpretation of lipid biomarker and stable isotope data were carried out by Dewi Ford, Dan Mayor and Anna K. Wadsworth at the University of Exeter. The raw data files include lipid biomarker and stable isotope data for all analysed macrobenthic invertebrates and calanoid copepods, while the processed data files include all calanoid copepod data but selected macrobenthic taxa only, being Amphipoda, Decapoda, Isopoda, Mysidacea, Holothuroidea, Ophiuroidea, and Chaetognatha. The contributions of KL, JF and DJM were supported by the BIOPOLE National Capability Multicentre Round 2 funding from the Natural Environment Research Council (grant no. NE/W004933/1). DJM also received funding from OceanICU (EU Grant agreement ID: 101083922; UKRI grant reference: 10063673). SB received support via the Leibniz foundation and the ALONGate project (A Long-term Observatory of the North Atlantic Gateway to the Arctic Ocean) via grant number PS150/2023 in the program for women professors. The field work for PS136 was supported by grant no. AWI_PS136_01. No credit. completed Linse, Katrin British Antarctic Survey Researcher author Ford, Dewi University of Exeter Researcher author Blackbird, Sabena J. University of Liverpool Researcher author Brix, Saskia Senckenberg am Meer Researcher author Freer, Jennifer J. British Antarctic Survey Researcher author Jeffreys, Rachel University of Liverpool Researcher author Schmidt, Lydia A. University of Rostock Researcher author Mayor, Daniel J. University of Exeter Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates EARTH SCIENCE > Oceans > Ocean Chemistry > Hydrocarbons EARTH SCIENCE > Oceans > Ocean Chemistry > Stable Isotopes EARTH SCIENCE > Oceans > Marine Environment Monitoring theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher δ15N signatures Calanus Macrobenthos benthopelagic coupling fatty acid and alcohol biomarkers theme Greenland Sea - Fram Strait - Molloy Deep Arctic place Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions public access limited according to Article 13(1)(e) of the INSPIRE Directive license Open Government Licence v3.0 otherRestrictions Dara are supplied under Open Government Licence v.3 otherRestrictions Data are embargoed until publication of Dewi et al. (submitted June 2025) or made freely available after 2 years (2027). url url crossReference url url crossReference doi doi crossReference textTable eng utf8 biota environment oceans -2.77913 6.14365 78.60286 79.61481 2023-05-29 2023-06-11 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/csv bytes 679936 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=e115d087-e3e4-4794-b4e0-18262b8e6400 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: The marine invertebrates were collected during the RV Polarstern expedition PS136. The pelagic copepods Calanus finmarchicus and Calanus hyperboreus were collected using a BONGO net and a Multi-net. Benthic and epibenthic marine invertebrates were collected using an epibenthic sledge. Specimens for lipid and bulks stable isotope analyses were sorted live, photographed and fixed -80 °C in cryovials. Lipid extractions and Gas chromatography-mass spectrometry (GC-MS) analyses were carried out in the Jeffreys' lab at the University of Liverpool following their standard protocol. Compounds were identified either by comparison of their mass spectra and relative retention indices with those available in the literature, and/or by comparison with authentic standards. Shorthand notations of fatty acids and alcohols follows the IUPAC (International Union of Pure and Applied Chemistry, hhtp://www.iupac.org) systematic nomenclature 'n-x' notation. Quantitative data were calculated by comparison of peak areas of the internal standard with those of the compounds of interest, using the total ion current (TIC) chromatogram. The relative response factors of the analytes were determined individually for 36 representative fatty acids and sterols using authentic standards. Response factors for analytes where standards were unavailable were assumed to be identical to those of available compounds of the same class. Bulk stable isotope analyses were carried out in the Jeffreys' lab at the University of Liverpool following their standard protocol. Isotope values were corrected using internationally certified isotopic reference materials USGS40 and USGS41a (Reston Stable Isotope Laboratory) and are reported in delta-notation (per mille) relative to atmospheric nitrogen (N2) and Vienna PeeDee Belemnite carbonate (V-PDB) scales. An internal standard of homogenized prawn (Penaeus vannamei) with well characterized d15N values (6.50 per mille +/- 0.16 per mille) was used to measure precision. In the data analyses Quantitative lipid constituent data were separated into three datasets which included lipid compound groups (e.g., saturated fatty acids, monounsaturated fatty acids), individual fatty acids, and individual fatty alcohols. These data were converted to relative abundance (mol%) of total identified lipid compounds, fatty acids, or fatty alcohols, respectively. The relative abundance of fatty acid isomers with the same number of carbon molecules and double bounds but unidentified omega groups were summed and labelled as 'X1:X2(iso)'. Data collection: The epibenthic sledge used for the collection of the benthic invertebrates was DZMB Senckenberg Wilhelmshaven build following the design by Brenke (2005). Gas chromatography-mass spectrometry (GC-MS) analyses of the total lipid extracts were conducted using a GC Trace 1300 fitted with a split-splitless injector and column DB-5MS. The eluent from the GC was transferred directly via a transfer line (320 degrees Celsius) to the electron impact source of a Thermoquest ISQMS single quadrupole mass spectrometer. Mass data were collected at a resolution of 600, cycling every second from 50 to 600 Da and were processed using Xcalibur software. Bulk stable isotope samples were analysed following acid vapour treatment (HCL; 12 h; Yamamuro and Kayanne, 1995), using an ECS8020 elemental analyser (NC Technologies) coupled to a Delta V isotope ratio mass spectrometer (Thermo Scientific). Data quality: The identification to species in the calanoid copepods were carried out by experienced zooplankton scientists, while the macrobenthic invertebrates were consistently identified to higher taxonomic levels (Phylum, class, order) but only selected specimens identified to Genus or species. Lipid and bulk stable isotope data were quality controlled using standards. asNeeded completed