BGS Hosted Metadata
Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle
The data presented are quantitative polymerase chain reaction (qPCR) read outs from antimicrobial resistance gene (AMRG) assays and associated metadata from this project. In this dataset, the mean gene copy numbers per microlitre of DNA extract are shown. The data were collected from faecal and environmental samples which were obtained from a single British commercial pig unit. The former were collected from the sow housing barn, pig growing houses and slurry tanks within the farm unit and the latter were obtained through random stratified sampling of the farm and the surrounding land. These samples were taken from what will be referred to as the 'main study'. A further study was carried out to obtain samples after a partial depopulation which took place on this farm. Faecal samples were obtained from the sow housing barn, pig growing houses and slurry tanks and will be referred to as the 'depopulation (depop) study'. For the main study, the samples were collected between October 19th 2016 and April 5th 2017. For the depop study, the samples were collected between 19th June 2017 and 13th November 2017. The data associated with all samples were generated between August 1st 2017 and May 1st 2018. Full details about this dataset can be found at https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
Simple
- Date (Publication)
- 2018-08-10
- Citation identifier
- https://catalogue.ceh.ac.uk/id/e548dc5d-49e3-467d-9435-c199da40e7be
- Citation identifier
- doi: / 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- Other citation details
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Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- Point of contact
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Organisation name Individual name Electronic mail address Role The Roslin Institute Pollock, J.
Point of contact SRUC/The Roslin Institute
Pollock, J. Author R(D)SVS/The Roslin Institute
Corbishley, A.
Author SRUC
Hutchings, M.R.
Author The Roslin Institute Gally, D.L.
Author NERC Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian University of Edinburgh EMAIL NOT PROVIDED
Owner
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GEMET - INSPIRE themes, version 1.0
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Soil
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Agricultural and Aquaculture Facilities
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Human Health and Safety
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- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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© University of Edinburgh
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Farming
- Begin date
- 2016-10-19
- End date
- 2017-04-05
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- Unique resource identifier
- OSGB 1936 / British National Grid
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/e548dc5d-49e3-467d-9435-c199da40e7be Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/e548dc5d-49e3-467d-9435-c199da40e7be.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Faecal and soil samples were collected from the study farm weekly using spooned universal tubes and were stored at -20 deg C, batch transported on dry ice to the laboratory where they were stored at -80 deg C until DNA extraction was carried out. DNA extracts were quantified and assessed using a NanoDrop spectrophotometer and split into aliquots for storage. DNA extracts were then screened in triplicate by qPCR and the number of gene copies assessed through the use of a standard curve generated by the inclusion of standards with known copy numbers of each target gene. Non-template controls were also included to ensure that non-specific binding or contamination were not occurring. The qPCR machine software (i.e. MxPro) was then used to calculate the number of gene copies in each of the collected samples and the mean was calculated using the three replicates. These values are included in the data set provided.
Metadata
- File identifier
- e548dc5d-49e3-467d-9435-c199da40e7be XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-03-21T13:21:52
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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