e65881be-9dca-4111-8a3b-fb84cf78cf15 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-21T16:52:26 UK GEMINI 2.3 Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids 2025-11-13 publication 2025-08-21 creation https://catalogue.ceh.ac.uk/id/e65881be-9dca-4111-8a3b-fb84cf78cf15 10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15 doi: Parker, M.E., Zytynska, S.E. (2025). Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids. NERC EDS Environmental Information Data Centre 10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15 [This nonGeographicDataset is embargoed until January 4, 2027]. This dataset comprises whole-leaf and headspace metabolomics of barley plants (Hordeum vulgare) that were root-inoculated with three rhizobacterial treatments: Acidovorax radicis N35, Bacillus subtilis B171, or no bacteria (control). Plants were also either infested with Sitobion avenae aphids or left uninfested as controls. Plants were grown under controlled conditions in glasshouse (leaf extracts) or climate chamber (headspace analysis). The purpose of these experiments was to compare metabolomic profiles across rhizobacterial treatments, and when aphids were feeding on plants, as an assessment of wider-community effects on rhizosphere inoculation of barley plants. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15 Sharon Zytynska University of Manchester sharon.zytynska@manchester.ac.uk pointOfContact Parker, M.E. University of Liverpool m.e.parker@liverpool.ac.uk https://orcid.org/0009-0007-4602-8382 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Zytynska, S.E. University of Manchester sharon.zytynska@manchester.ac.uk https://orcid.org/0000-0002-0174-3303 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author University of Liverpool EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher plant ecology chemical analysis insect predator GEMET - Concepts, version 4.1.3 2019-01-28 creation Biodiversity Agriculture Ecosystem services otherRestrictions Embargoed otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Parker, M.E., Zytynska, S.E. (2025). Metabolomic data from the whole-leaf and headspace of barley plants inoculated with rhizobacteria and infested with aphids. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/e65881be-9dca-4111-8a3b-fb84cf78cf15 English utf8 biota Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Barley plants (Hordeum vulgare, cultivars Irina (KWS seeds) and Firefoxx (Elsoms seeds)), were treated with Acidovorax radicis N35, Bacillus subtilis B171, or a control, and either infested with Sitobion avenae aphids or left uninfested. In the first experiment, whole leaves were sampled, metabolites extracted with hexane, derivatised and analysed using an Agilent 7250 GC-qToF-MS equipped with an HP-5MS column (30 m × 0.25 mm × 0.25 μm). In the second experiment, plants were enclosed in transparent PET film bags (31 cm × 30 cm; Toppits® Bratschlauch, Melitta Group, Germany) sealed at both ends and headspace volatiles were collected using Tenax probes and analysed on a GC-qToF-MS coupled with thermal desorption. Data were processed using MS-DIAL, with blank subtraction, internal standard normalisation, QC correction, and log transformation. Annotated peaks were matched against spectral libraries. Features were assigned metabolite numbers to enable unbiased comparisons, including unknowns, across both experiments.