BGS Hosted Metadata
E. coli counts of fresh cattle faeces from the North Wyke Farm Platform
The data consists of faecal microbiology and moisture content taken from two separate cohorts of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected between November 2016 and July 2018. Samples were collected and microbiologically analysed in the laboratory within 6 to 8 hours. Two cohorts of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Each cohort covering the period that cattle enter the farm platform, i.e. from weaning until slaughter ca. 16 – 20 months. Full details about this dataset can be found at https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
Simple
- Date (Publication)
- 2019-09-13
- Citation identifier
- https://catalogue.ceh.ac.uk/id/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- Citation identifier
- doi: / 10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- Other citation details
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Hodgson, C.J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre 10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- Point of contact
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Organisation name Individual name Electronic mail address Role Rothamsted Research Hodgson, C.J. Author Rothamsted Research Hodgson, C.
Point of contact NERC EDS Environmental Information Data Centre Custodian NERC Environmental Information Data Centre Publisher
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GEMET - INSPIRE themes, version 1.0
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Environmental Monitoring Facilities
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Wikidata
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- no limitations
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© Natural Environment Research Council
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If you reuse this data, you should cite: Hodgson, C.J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- Spatial representation type
- Text, table
- Distance
- 5 urn:ogc:def:uom:EPSG::9001
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Farming
- Environment
- Begin date
- 2016-11-15
- End date
- 2018-07-17
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- Unique resource identifier
- WGS 84
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/ec83a4ea-923a-4f02-8a9f-aeecc43d7123.zip Supporting information
- Hierarchy level
- Dataset
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory one gram of faecal material was aseptically transferred to nine ml of sterile Ringers (Oxoid, Basingstoke, UK) and appropriate serial 10-fold dilutions were made. An aliquot (0.1 ml) was plated in duplicate on to Membrane Lactose Glucuronide Agar (MLGA) (Oxoid) and spread across the surface of the agar using a disposal plastic spreader (VWR). Antibiotics were added to the MLGA at suitable concentrations after autoclaving and prior to pouring the plates. Antibiotics were added at the following rates; 16 mg/l for tetracycline and cephalexin, 1.0 mg/l marbofloxacin, 8 mg/l meropenem. All plates were allowed to air dry prior to inversion and incubation, at 44.5 ◦C (±0.2 ◦C) for 18–24 h. Colonies were counted, after incubation, and reported as E.coli colony forming units (CFU) per gram fresh weight and also on a dry weight basis. All colony counts were further transformed by log to base 10. Gravimetric moisture content of faecal samples, approximately 50 g of faecal material was accurately weighed into a pre-weighed silver foil container and dried in an oven at 105 ◦C, until constant weight. To calculate % dry matter: 100 – (((fresh wt.-Dry wt.) /(fresh wt.))*100), effectively 100 – moisture content. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.
Metadata
- File identifier
- ec83a4ea-923a-4f02-8a9f-aeecc43d7123 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-11-13T16:15:32
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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