eef3a20c-cb4c-415f-b4be-4d1c31fb6015 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2026-02-06T13:36:12 UK GEMINI 2.3 Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions 2024-10-28 publication https://catalogue.ceh.ac.uk/id/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 doi: Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre 10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 This dataset contains information about the number of bacterial colonies recovered from plastics beads that were incubated in a 96-well plate for 24 hours at 37 degrees Celsius. Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control. The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 McCarthy, R.R. Brunel University London Ronan.mccarthy@brunel.ac.uk pointOfContact Howard, S. Brunel University London Sophie.howard@brunel.ac.uk author McCarthy, R.R. Brunel University London Ronan.mccarthy@brunel.ac.uk author Brunel University London EMAIL NOT PROVIDED owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Human Health and Safety theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Pollution Biofilm plastic degradation polyesterase Escherichia coli otherRestrictions no limitations otherRestrictions This resource is licensed under a Creative Commons Attribution 4.0 International Licence otherRestrictions If you reuse this data, you should cite: Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 English utf8 health Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/eef3a20c-cb4c-415f-b4be-4d1c31fb6015.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted.