eef71670-f6dc-46f6-9143-7569599854e5 eng utf8 dataset dataset NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom polardatacentre@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact 2024-05-22 ISO 19115 Geographic Information - Metadata ISO 19115:2003(E) European Petroleum Survey Group (EPSG) Geodetic Parameter Registry 2008-11-12 publication European Petroleum Survey Group EPSGadministrator@iogp.org https://www.epsg-registry.org/ EPSG Geodetic Parameter Dataset The EPSG Geodetic Parameter Dataset is a structured dataset of Coordinate Reference Systems and Coordinate Transformations, accessible through this online registry. information publisher urn:ogc:def:crs:EPSG::3031 6.18.3 Dissolved nutrient and particulate material concentrations and phytoplankton abundance and community composition from cruise JC211 to South Georgia, Southern Ocean, February 2021 2024-05-22 creation 2024-05-22 revision 2024-05-22 publication 2024-05-22 released 1.0 https://doi.org/10.5285/eef71670-f6dc-46f6-9143-7569599854e5 doi GB/NERC/BAS/PDC/01852 https://data.bas.ac.uk/ NE/N018095/1 award NE/V013254/1 award NE/K010034/1 award Please cite this item as: Abrahamsen, P., Tarling, G., Firing, Y., King, B., Marzocchi, A., Burson, A., Hendry, K., Henley, S., Liszka, C., Manno, C., Ward, F., Woodward, M., Wootton, M., Brunetta, M., Song, Y., & Thorpe, S. (2024). Dissolved nutrient and particulate material concentrations and phytoplankton abundance and community composition from cruise JC211 to South Georgia, Southern Ocean, February 2021 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/eef71670-f6dc-46f6-9143-7569599854e5 Marine macronutrient and particulate material concentrations together with phytoplankton abundance and community composition were measured from samples taken during British Antarctic Survey and UK National Oceanography Centre research cruise JC211 to the Scotia Sea, Southern Ocean, carried out onboard RRS James Cook in February-March 2021. Samples were taken from four sections of the cruise: (i) at the British Antarctic Survey Scotia Sea Open-Ocean Observatory (SCOOBIES) P3 mooring in the Georgia Basin, northwest of South Georgia; (ii) as part of the British Antarctic Survey long-term Polar Ocean Ecosystem Time Series - Western Core Box (POETS-WCB) survey at South Georgia; (iii) in the vicinity of giant iceberg A-68A and associated icebergs; and (iv) as part of the A23 repeat hydrographic section. Samples were collected to maximum depth of approximately 500 m from Niskin water bottles attached to a CTD rosette. Full data analyses were performed post-cruise. Concurrent temperature, salinity, dissolved oxygen, chlorophyll fluorescence and photosynthetically active radiation (PAR) measurements obtained from analysis of water samples and from sensors on the CTD system at the depth and time of each water sample are provided for environmental context. RRS James Cook cruise JC211 was in part supported by the Natural Environment Research Council (NERC) National Capability Science (Antarctic Logistics and Infrastructure; NC-ALI) programme. Further funding for sampling around iceberg A-68 was provided by the Government of South Georgia and the South Sandwich Islands and the UK Government Blue Belt Programme. Data acquisition and analyses were supported by NERC NC-ALI funding to the Ecosystems CONSEC Programme and NERC Grants NE/N018095/1 (ORCHESTRA) and NE/V013254/1 (ENCORE) at the British Antarctic Survey, and by the European Research Council (ERC Starting Grant 678371 ICY-LAB to K Hendry) and NERC Grant NE/K010034/1 (to SF Henley). No credit. completed Abrahamsen, Povl British Antarctic Survey Researcher author Tarling, Geraint A British Antarctic Survey Researcher author Firing, Yvonne L National Oceanography Centre Researcher author King, Brian A National Oceanography Centre Researcher author Marzocchi, Alice National Oceanography Centre Researcher author Burson, Amanda British Antarctic Survey Researcher author Hendry, Katharine R British Antarctic Survey Researcher author Henley, Sian F University of Edinburgh Researcher author Liszka, Cecilia M British Antarctic Survey Researcher author Manno, Clara British Antarctic Survey Researcher author Woodward, Malcolm Plymouth Marine Laboratory Researcher author Wootton, Marianne Marine Biological Association of the United Kingdom Researcher author Brunetta, Martina Marine Biological Association of the United Kingdom Researcher author Song, Yan University of Bristol Researcher author Thorpe, Sally E British Antarctic Survey Researcher author NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information pointOfContact asNeeded completed EARTH SCIENCE > Oceans > Ocean Chemistry > Ammonia EARTH SCIENCE > Oceans > Ocean Chemistry > Nitrate EARTH SCIENCE > Oceans > Ocean Chemistry > Nitrite EARTH SCIENCE > Oceans > Ocean Chemistry > Organic Carbon EARTH SCIENCE > Oceans > Ocean Chemistry > Phosphate EARTH SCIENCE > Oceans > Ocean Chemistry > Silicate EARTH SCIENCE > Oceans > Marine Environment Monitoring theme Global Change Master Directory (GCMD) Science Keywords 2026-01-27 publication 23 Global Change Data Center, Science and Exploration Directorate, Goddard Space Flight Center (GSFC) National Aeronautics and Space Administration (NASA) Greenbelt MD United States of America https://earthdata.nasa.gov/about/gcmd/global-change-master-directory-gcmd-keywords Global Change Master Directory (GCMD) Keywords The information provided on this page seeks to define how the GCMD Keywords are structured, used and accessed. It also provides information on how users can participate in the further development of the keywords. information publisher Antarctica South Georgia Southern Ocean iceberg, A-68 nutrients particulate nitrogen particulate organic carbon particulate silica phytoplankton abundance phytoplankton community composition theme Scotia Sea, South Georgia Southern Ocean place Oceanographic geographical features theme GEMET - INSPIRE themes, version 1.0 2021-01-06 revision otherRestrictions no limitations to public access otherRestrictions no limitations license Open Government Licence v3.0 otherRestrictions This data is governed by the NERC data policy and supplied under Open Government Licence v.3 otherRestrictions None. doi doi crossReference doi doi crossReference doi doi crossReference url url crossReference url url crossReference url url crossReference url url crossReference doi doi crossReference url url crossReference url url crossReference url url crossReference url url crossReference url url crossReference url url crossReference textTable eng utf8 biota environment oceans -43.781 -34.4025 -57.127 -55.221 2021-02-04 2021-02-17 It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk. NERC EDS UK Polar Data Centre +44 (0)1223 221400 British Antarctic Survey, High Cross, Madingley Road Cambridge Cambridgeshire CB3 0ET United Kingdom PDCServiceDesk@bas.ac.uk https://www.bas.ac.uk/team/business-teams/information-services/uk-polar-data-centre/ information distributor text/plain text/csv bytes 47309 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=eef71670-f6dc-46f6-9143-7569599854e5 WWW:LINK-1.0-http--link Get Data Download data download bytes 47309 http://ramadda.data.bas.ac.uk/repository/entry/show?entryid=eef71670-f6dc-46f6-9143-7569599854e5 WWW:LINK-1.0-http--link Get Data Download data download dataset Methodology: Water samples were taken from 10 L Niskin bottles mounted on the CTD rosette during cruise JC211. Only selected water samples were analysed for the variables in this dataset; NaN denotes samples where particular analyses were not performed. Nutrient samples were taken over the upper 500 m of each CTD cast at intervals that were larger in the deeper parts of each profile and smaller in surface waters, with 5 ± 1 depths sampled per profile. Samples were taken in acid cleaned 60 ml HDPE bottles and frozen at -20°C for subsequent analysis on shore at Plymouth Marine Laboratory. Samples were thawed using International GO-SHIP protocols (Becker et al., 2020) to allow redissolution of any secondary silicate precipitates that formed during freezing. Samples were analysed using a SEAL analytical AAIII segmented flow colorimetric autoanalyser for nitrate+nitrite, nitrite, phosphate, silicic acid and ammonium, following Woodward and Rees (2001). Sample handling and analytical protocols followed the International GO-SHIP protocols (Becker et al., 2020) as closely as possible. Water samples for particulate organic carbon (POC) and particulate nitrogen (PN) analysis were taken from the CTD at two depths (200 m and 400 m). 250 ml water was collected directly into a HDPE bottle and immediately frozen at -20 °C. The water samples were filtered onto pre-combusted (450 °C, 16 h) 25 mm glass fibre filters (GF/F; nominal pore size 0.7 µm) and rinsed with water purified using a Millipore Milli-Q lab water system. Samples were air-dried and fumed for 24 h with 37 % HCl in a desiccator before finally being oven-dried at 50 °C for 24 h. Filters and filter blanks were placed in sterile tin capsules, and POC and PN were measured on a CE440 Elemental Analyser (Exeter Analytical Limited, Coventry, UK), calibrated using an acetanilide calibration standard with a known % C and % N of 71.09 % and 10.36 % respectively. Standards were interspersed regularly between samples to measure and correct for drift. Water samples for particulate silica (SiO2) analysis were taken from the CTD at three depths (surface at either 5 m or 10 m, the chlorophyll a maximum which varied between 10 m and 50 m, and 100 m). Bottle material was filtered onto 25 mm, 0.4 µm polycarbonate filters and rinsed with Milli-Q water before drying at 50 °C for 24 h. Material on the filters was solubilised via an alkaline extraction method (Hatton et al., 2019) carried out at the Bristol Isotope Group laboratory. Sample material was digested in Teflon tubes with 0.2M NaOH at 100 °C for 40 min. This was followed by neutralisation with 6M HCl. Particulate silica concentrations were measured colorimetrically by spectrophotometry (heteropoly blue method; Strickland and Parsons, 1972) using a Hach DR3900 spectrophotometer set at a wavelength of 815 nm calibrated against a NIST silica standard. Water samples for phytoplankton analysis were taken from the CTD at four depths corresponding to near surface (5 or 10 m), the chlorophyll a maximum (25 m for all stations used in this analysis other than station H2, which was 10 m), 100 m and 200 m. 200 ml water was collected directly into an amber glass bottle at each depth and was fixed immediately with 4 ml Lugol's iodine solution. Bottles were gently mixed and stored in the dark at 4 °C until later analysis. A sub-sample of phytoplankton samples from the surface (5 m) and chlorophyll a maximum were analysed for phytoplankton and microzooplankton (heterotrophic organisms 20-200 µm in size). Cell identification and enumeration were carried out using standard Utermöhl methodology (Karlson et al., 2010). Subsamples of 50 ml were settled for 24 hours and examined under an inverted light microscope, with taxa identified to species or genera where possible, or by group or size otherwise. Where chains were encounte...(4) Data collection: 10 L OceanTest Equipment model 110B Niskin bottles mounted on the CTD frame were used to take discrete water samples for chemical and biological analysis. Nutrient samples were analysed using a SEAL analytical AAIII segmented flow colorimetric autoanalyser for nitrate+nitrite, nitrite, phosphate, silicic acid and ammonium, following Woodward and Rees (2001). Particulate organic carbon (POC) and particulate nitrogen were measured on a CE440 Elemental Analyser (Exeter Analytical Limited, Coventry, UK). Particulate silica concentrations were measured colorimetrically by spectrophotometry (heteropoly blue method; Strickland and Parsons, 1972) using a Hach DR3900 spectrophotometer set at a wavelength of 815 nm calibrated against a NIST silica standard. Phytoplankton cell identification and enumeration were carried out using standard Utermohl methodology (Karlson et al., 2010). A Sea-Bird Scientific SBE 911plus Conductivity-Temperature-Depth (CTD) system was used to obtain profiles of temperature, salinity, dissolved oxygen (SBE 43 dissolved oxygen sensor), fluorescence (Chelsea Technologies AquaTracka Mk III) and photosynthetically active radiation (PAR; Biospherical QCP-2350-HP). A Sea-Bird Scientific SBE 35 deep ocean standards thermometer was mounted on the CTD frame, taking a measurement each time a bottle was closed. Discrete water samples for salinity and dissolved oxygen calibration of the CTD data were collected from a subset of Niskin bottles. Salinity samples were analysed on a Guildline Autosal 8400B salinometer against IAPSO standard seawater batch P164. Dissolved oxygen samples were collected and analysed on board following GO-SHIP procedures (Langdon, 2010), using a Metrohm automatic titration system with an amperometric system to determine the endpoint of the Winkler titration. Data quality: Nutrient calibration standards were prepared with low-nutrient seawater and the analyses were quality controlled and checked using Certified Reference Materials (CRMs) for nutrients in seawater from KANSO Ltd. (Japan). Raw data were further corrected to ambient ocean salinity and pH. Samples were analysed in duplicate and standard deviation was generally better than 0.02 µmol L-1 for nitrate+nitrite, 0.01 µmol L-1 for nitrite, 0.02 µmol L-1 for phosphate, 0.06 µmol L-1 for silicic acid and 0.05 µmol L-1 for ammonium. Analytical precision was better than 1.0 % for particulate organic carbon (POC) and 1.1 % for particulate nitrogen. Analytical precision for particulate silica was better than 3 %, and full replicates of sample aliquots taken through the dissolution process separately agreed within 8 %. Laboratory blanks were below the detection limit (0.01 mg L-1). The standard deviation in practical salinity differences between the bottle and sensor values was less than 0.0015. Dissolved oxygen samples had residual errors, and differences between downcasts and upcasts, generally within 1 µmol kg-1. Chlorophyll fluorescence data from the CTD had the factory calibration applied, but no calibration against in situ samples. NaN is used to indicate any bottle samples where a particular parameter was not measured. The following variables have WOCE data quality flags of 2 (acceptable measurement), 3 (questionable measurement), 4 (bad measurement), 5 (not reported), 9 (not sampled): - Salinity from sample measurement - Dissolved oxygen from sample measurement Manual flagging was not performed for the salinity samples; the flags merely indicate the presence (or absence) or samples. For dissolved oxygen samples, the use of these flags is described in more detail in the cruise report (Abrahamsen, 2021). asNeeded completed