Date (Creation)

2022-01-13

Date (Revision)

2022-01-13

Date (Publication)

2022-01-13

Date (released)

2022-01-13

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/eefba4cd-c7a3-4e24-b2c8-5221fcfdde0b

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01601

Codespace

https://data.bas.ac.uk/

Unique resource identifier

NE/R012733/1

Codespace

award

Other citation details

Please cite this item as: Grigor, J., Freer, J., Cohen, J., Tarling, G., & Last, K. (2022). Morphometric, swimming activity and raw oxygen air saturation data from experiments on CV Calanus finmarchicus in the Fram Strait, Arctic Ocean, in August 2019 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/eefba4cd-c7a3-4e24-b2c8-5221fcfdde0b

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
Scottish Association For Marine Science	Grigor, Jordan		Author
British Antarctic Survey	Freer, Jennifer J		Author
University of Delaware	Cohen, Jon		Author
British Antarctic Survey	Tarling, Geraint A		Author
Scottish Association For Marine Science	Last, Kim		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
• EARTH SCIENCE > Oceans > Marine Environment Monitoring

Theme

• Arctic Ocean

• Calanus finmarchicus

• copepod

• diapause

• swimming activity

• torpidity

Place

• Greenland Sea, Fram Strait Arctic Ocean

GEMET - INSPIRE themes, version 1.0

• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Data released under Open Government Licence V3.0:

Use constraints

Other restrictions

Other constraints

None.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Larger work citation

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Environment
• Oceans

Begin date

2019-08-13

End date

2019-08-20

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Morphometric data:

Collected for 417 Calanus finmarchicus copepods (stage CV) collected in the Fram Strait, Arctic Ocean. Swimming activity in the laboratory was measured in 225 epipelagic and 192 mesopelagic copepods from two stations (F7 and D6) in August 2019, coinciding with the timing of early diapause in this species. Copepods were photographed under a stereomicroscope after removal from the locomotor activity monitor experiments (see swimming activity dataset). Photographs were taken onboard RRS James Clark Ross. The following measurements were made; body size (prosome areas in mm2) lipid sac area, as well as lateral lipid sac area / prosome area (LA / PA ratios). Data are thus separated for copepods from each activity monitor allowing pairing with the other dataset.

Copepods were sourced from epipelagic and mesopelagic depths for comparisons of diapause indicators at stations F7 and D6 in the Fram Strait (Greenland Sea). Copepods were collected from the water column using the Hydrobios® Mammoth MultiNet system, comprising nine individual nets (1 m2 apertures, 300 µm mesh) that were towed vertically at 10-20 m min-1 (ascent speed). Upper mesopelagic (285-520 m) and epipelagic (5-170 m) depth ranges were sampled. These copepods were first used in activity experiments lasting 6-day periods. They were identified as CV C. finmarchicus using morphological approaches prior to these experiments, and their identifications were since confirmed using molecular approaches. Photographs were taken at 25 objective magnification using a WILD microscope. A calibration ruler allowed pixel-mm conversions. Back on land, the photographs were analysed using ImageJ (FIJI) for the following measurements: body size (prosome areas in mm2), lipid sac area, and lipid sac / prosome area (LA / PA) ratios.

Swimming activity data:

Collected using locomotor activity monitors for 469 Calanus finmarchicus copepods (stage CV) collected in the Fram Strait, Arctic Ocean. Swimming activity in the laboratory was measured in 246 epipelagic and 223 mesopelagic copepods from two stations (F7 and D6) in August 2019, coinciding with the timing of early diapause in this species. Individual copepod swimming activity was monitored every minute (here binned into 30-minute bins for analysis), for six days using modified LAM10 activity monitors (Trikinetics®). These are infrared light beam arrays which detect the movement of individual copepods in test chambers (clear acrylic tubes of 10 mm diameter and filled with 0.2 µm filtered seawater), registering beam break events on a laptop computer using proprietary Trikinetics® software (filescan.exe). Experiments were conducted in darkness at two experimental temperatures in an incubator onboard RRS James Clark Ross. Copepods were sourced from epipelagic and mesopelagic depths for comparisons of diapause indicators at stations F7 and D6 in the Fram Strait (Greenland Sea). Copepods were collected from the water column using the Hydrobios® Mammoth MultiNet system, comprising nine individual nets (1 m2 apertures, 300 µm mesh) that were towed vertically at 10-20 m min-1 (ascent speed). Upper mesopelagic (285-520 m) and epipelagic (5-170 m) depth ranges were sampled. CV C. finmarchicus copepods were then identified in darkness using taxonomic identifications, later verified by molecular tools. Experiments were conducted over 6-day periods. Activity monitors recorded infra-red beam breaks by individual copepods at two positions (middle and bottom) inside acrylic tubes every 60 seconds. Experiments were conducted in darkness at 2.8oC (station F7) and 4oC (station D6), consistent with environmental temperatures at the collection depths.

Oxygen consumption activity data:

Collected using a Loligo microplate respirometry system for 40 Calanus finmarchicus copepods (stage CV) collected in the Fram Strait, Arctic O...(28)

Data collection:

Photographs were taken using a stereomicroscope onboard the ship. Fiji was used as the image analysis software ( https://imagej.net/software/fiji).

Swimming activity was measured using modified LAM10 activity monitors (Trikinetics), infrared light beam arrays which detect the movement of an individual copepod in the middle of a test chamber (clear acrylic tubes of 10 milimetre diameter and filled with 0.2 micrometre filtered seawater). Beam break events were registered on a laptop computer using proprietary Trikinetics software (filescan.exe).

Oxygen consumption was measured in 10 individuals from each depth layer (20 individuals total from each station) for 2 days, using a Loligo microplate respirometry system (1700 microlitre well plates containing 0.2micrometre filtered seawater), calibrated at 4 degrees Celsius.

Metadata

File identifier

eefba4cd-c7a3-4e24-b2c8-5221fcfdde0b XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2022-01-13

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact