efcef5e3-09f2-4a23-ad19-53295a3171ce English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:24:19 UK GEMINI 2.3 OSGB 1936 / British National Grid Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform 2020-05-18 publication https://catalogue.ceh.ac.uk/id/efcef5e3-09f2-4a23-ad19-53295a3171ce 10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce doi: Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre 10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce The data consists of the abundance of antibiotic resistant genes (ARG) in fresh cattle faeces from faecal samples taken from a cohort of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected in November 2016. The cohort of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Samples were collected from cattle as they entered housing for the winter, i.e. at weaning. Full details about this dataset can be found at https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce Hodgson, C.J. Rothamsted Research North Wyke chris.hodgson@rothamsted.ac.uk https://orcid.org/0000-0003-4067-3210 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Hodgson, C. Rothamsted Research North Wyke chris.hodgson@rothamsted.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher Rothamsted Research comms@rothamsted.ac.uk owner Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Antibiotic resistant gene abundance North Wyke Farm Platform otherRestrictions no limitations otherRestrictions © Natural Environment Research Council otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Hodgson, C.J. (2020). Antibiotic resistant gene abundance in fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/efcef5e3-09f2-4a23-ad19-53295a3171ce textTable 5 English utf8 biota environment 2016-11-01 2016-11-30 -3.93 -3.88 50.74 50.77 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/efcef5e3-09f2-4a23-ad19-53295a3171ce Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/efcef5e3-09f2-4a23-ad19-53295a3171ce.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory faecal samples were pooled, such that for each farmlet (10 cattle) there were 3 samples, (3, 3, 4 cattle). This resulted in 9 faecal samples from which DNA was extracted using a commercially available extraction kit (MO BIO PowerSoil® DNA Isolation kit (catalogue No. 12888-100). The following adaption to the Power Soil protocol; at sample preparation samples were heated to 65 °C for 10 minutes prior to vortex. DNA was sequenced by the sequencing unit at the University of Exeter. The resulting metagenomic data was analysed at the Environmental Biotechnology Laboratory at the University of Hong Kong. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.