BGS Hosted Metadata
Obesogens in Scottish grey seals 2015-2018
The impacts of Persistent Organic Pollutants (POPs) are assessed in fat tissue (blubber) using live explant samples from young grey seals (Halichoerus grypus) on the east coast of Scotland. The data show POP levels in blubber and responses to intrinsic pollutants and experimental exposure to polychlorinated biphenyls (PCBs), relevant due to its applicability to other species, including humans. The study sites are located on the Isle of May (56° 10’ 57” N, 02° 33’ 17” W) between October and December 2015, 2016, and 2017, where the grey seal population had been monitored annually and from where previous POP data from blubber tissue was investigated in 2002. A second sample set was collected from yearling grey seals hauled out on the Isle of May, Moray Firth, and Culbin Sands (57° 39’10” N, 03° 43’ 15” W) and maintained in temporary captivity at the Sea Mammal Research Unit between February and August 2018. The capture and explant data includes animal identifier, moulting state (where relevant for yearlings), whether the animal was fed or fasted (where relevant for pups), body and sample tissue mass. Characteristics of the animals like sex, birth, and weaning date (where known), rate of mass change, life history stage and blubber fat content, including intrinsic blubber POP levels, were measured at the Centre for Analytical Research and Technology at the University of Liège, Belgium to be used as covariates in a Generalised Additive Mixed Model (GAMM). This analysis was undertaken after accelerated solvent extraction of the samples in hexane dichloromethane (Dionex 200 by Thermo Fisher Scientific) and the extracts were analysed using a gas chromatograph (Agilent 6890 GC) coupled to Autospec Ultima High-Res Mass Spectrometer. The response of explants was tested through measurement of gene expression (using the Stratagene Mx3000P qPCR System or StepOne Plus Real-Time PCR System), insulin signaling and glucose uptake, lactate production, oxygen consumption and lipolytic rate using Randox Monza assays. Thyroid hormones triiodothyronine (TT3) and thyroxine (TT4) were measured using Enzyme Linked Immunosorbent Assays (ELISA Fortress Diagnostics Ltd). The live explants were treated overnight with a mix of PCBs and organochlorine pesticide derivatives (DDT, DDE), which were associated with altered metabolic function symptoms such as glucose uptake and lactate production in 2015-2017. The data will facilitate an understanding of the molecular mechanisms of contaminant-induced disruption of energy balance by legacy pollutants in seals during their most vulnerable life stages. The data collection was led by Dr Kimberley Bennett from Abertay University and funded by the NERC Discovery Science project “Obesogens in a naturally obese animal: An experimental approach to assess the impact of marine pollutants on fat tissue function in seals”, parent grant reference NE/M013723/1 with child grant NE/M01357X/1.
Simple
- Alternate title
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British Oceanographic Data Centre record 1048NE_M013723_1
- Date (Publication)
- 2024-07-05
- Date (Creation)
- 2024-07-05
- Date (Revision)
- 2024-07-05
- Citation identifier
- http://www.bodc.ac.uk/ / EDMED7345
- Point of contact
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Organisation name Individual name Electronic mail address Role Sea Mammal Research Unit
Unknown
Owner Sea Mammal Research Unit
Unknown
Originator British Oceanographic Data Centre
Custodian British Oceanographic Data Centre
Distributor
- Maintenance and update frequency
- As needed
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MEDIN metadata record availability
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GEMET - INSPIRE themes, version 1.0
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SeaDataNet Parameter Discovery Vocabulary
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SeaVoX Vertical Co-ordinate Coverages
- Access constraints
- Other restrictions
- Other constraints
- No limitations apply
- Other constraints
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Data are freely available to all following agreement to the terms and conditions of a Data Licence
- Use constraints
- Other restrictions
- Other constraints
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Usage restrictions are specified in the terms of the licence
- Spatial representation type
- Text, table
- Language
- English
- Topic category
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- Oceans
- Biota
- Environment
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- Geographic identifier
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Cromarty Firth
SeaVoX water bodies 2023-09-13 revision
- Geographic identifier
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Firth of Forth
SeaVoX water bodies 2023-09-13 revision
- Begin date
- 2015-10-23
- End date
- 2018-06-21
- Unique resource identifier
- urn:ogc:def:crs:EPSG::4326
- Codespace
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OGP
- Distribution format
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Name Version Delimited
- OnLine resource
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Protocol Linkage Name https://www.bodc.ac.uk/ Dataset Web Site
- Hierarchy level
- Dataset
Conformance result
- Title
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COMMISSION REGULATION (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Explanation
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BODC protocols are based on the Open Archival Information System (OAIS) model enabling BODC to iterate towards compliance with the on-going evolution and development of community requirements including FAIR (Findable,Accessible,Interoperable,Reusable), TRUST (Transparency, Responsibility, User community, Sustainability, Technology) and CARE (Collective Benefit, Authority to Control, Responsibility, Ethics). Data managers quality assure submissions and assemble the metadata necessary for curation. Submissions (as received) are placed in a long-term accession and stored in triplicate across multiple sites. Appropriate data are transferred into a standard internal format with source variable names mapped to controlled vocabularies, documentation assembled, and metadata loaded into BODC databases. Access to these data is through direct request, the BODC website and through partner repositories such as SeaDataNet. Access control is attained by assigning a data policy to each set of data and this policy is used to administer access when data are requested. Discovery metadata is aligned with EU INSPIRE (through MEDIN) and SeaDataNet community standards. Data are converted to open community formats including Ocean Data View ASCII and SeaDataNet NetCDF, with data described using terms from the NERC vocabulary server. BODC submission agreements are documented on the BODC website and customer service is assured with a dedicated requests team that serve data following local regulations including General Data Protection Regulation (GDPR) 2018 and Environmental Information Regulations (EIR) 2004.
- Pass
- Yes
- Statement
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The datasets cover measurements of persistent contaminants in seal blubber in two grey seals (Halichoerus grypus) populations on the Isle of May in Moray Firth and Culbin Sands, Scotland. Captured specimens were classified depending on animal characteristics like moulting state, whether the animal was fed or fasted, body and sample tissue mass. Blubber explants were collected in 2015-2018 and tested in Liège at the Centre for Analytical Research and Technology. Samples from different specimens were used as covariates in a Generalised Additive Mixed Model to assess the impact of Persistent Organic Pollutants (POPs) on metabolic and energy regulating functions such as thyroid hormone levels, glucose uptake and lactate production. The datasets were produced by researchers from Abertay University and funded by the NERC Discovery Science project, parent grant reference NE/M013723/1 with child grant NE/M01357X/1. The data were then provided to the British Oceanographic Data Centre (BODC) for archival and discoverability. No data quality or data processing procedures were applied by BODC.
Instrument(s) used to collect data: colorimeters; spectrophotometers.
Metadata
- File identifier
- f6589c51b2245d379b196603da773174 XML
- Metadata language
- English
- Hierarchy level
- Dataset
- Date stamp
- 2024-07-05T17:01:23
- Metadata standard name
- MEDIN
- Metadata author
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Organisation name Individual name Electronic mail address Role British Oceanographic Data Centre
Polly Hadziabdic
Point of contact
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