fdd72810-a57f-444f-83cf-f2351ef4c940 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:16:05 UK GEMINI 2.3 OSGB 1936 / British National Grid Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022 2024-08-09 publication https://catalogue.ceh.ac.uk/id/fdd72810-a57f-444f-83cf-f2351ef4c940 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940 doi: Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940 This dataset holds realistic environmental concentrations of potential pathogens colonising microplastic beads (nurdles) collected from ten Scottish beaches. Observed measures include concentrations and the present genes of Klebsiella bacteria isolated from the nurdles, as well as the salinity, electrical conductivity, turbidity, and polymer composition of the nurdles. In addition, an infection experiment was conducted to measure the virulence of the Klebsiella bacteria. Galleria melonella larvae were infected with the isolated Klebsiella bacteria and the number of living larvae was recorded. Full details about this dataset can be found at https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940 Metcalf, R. University of Stirling rebecca.metcalf@stir.ac.uk https://orcid.org/0000-0002-1463-427X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Messer, L. University of Stirling Lauren.messer@stir.ac.uk author White, H.L. University of Stirling Hannah.white@stir.ac.uk author Ormsby, M. University of Stirling Michael.ormsby1@stir.ac.uk author Matallana-Surget, S. University of Stirling Sabine.matallanasurget@stir.ac.uk author Quilliam, R. University of Stirling Richard.quilliam@stir.ac.uk https://orcid.org/0000-0001-7020-4410 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Metcalf, R. University of Stirling rebecca.metcalf@stir.ac.uk pointOfContact University of Stirling rebecca.metcalf@stir.ac.uk owner NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Environmental survey Pollution public health plastic pollution human pathogens plastisphere antimicrobial resistance virulence otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940 textTable English utf8 environment 2022-11-01 2022-11-30 -7.796 -1.081 54.677 58.005 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/fdd72810-a57f-444f-83cf-f2351ef4c940 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/fdd72810-a57f-444f-83cf-f2351ef4c940.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Microplastic beads were collected from ten Scottish beaches (Erskine, Yellowcraig, Largs, Irvine, Ayr, Turnberry, Montrose, Broughty Ferry, Portobello and Eyemouth) and bacteria were removed for quantification of target bacteria. Bacteria were quantified using selective media and positive colony-forming units (CFU) were enumerated. Water temperature and salinity at each site were also measured with a thermometer and a salinity refractometer (RGBS). Fourier Transform Infrared Spectrometry (FTIR) was conducted on a Thermo Scientific Nicolet is50 FTIR to identify the polymer composition of the nurdles. The virulence of the isolated Klebsiella was studied for one of the sites, Brought Ferry. Galleria mellonella model of infection was used to determine the virulence of each of the selected Klebsiella strains following their isolation from the microplastic beads and sand. After injection, larvae were incubated at and inspected 24, 48 and 72 h post-injection; larvae were considered dead when they did not respond to touch stimuli.