fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 English ISO/IEC 8859-1 (also known as Latin 1) nonGeographicDataset nonGeographicDataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:24:00 UK GEMINI 2.3 Measurements of respiration, incorporation of litter carbon into soil fractions, and microbial community data using mineral-amended soils 2025-10-22 publication 2025-10-22 creation https://catalogue.ceh.ac.uk/id/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 10.5285/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 doi: Elias, D.M.O., Mason, K.E., Goodall, T., Taylor, A., Zhao, P., Farina, A.O., Chen, H., Peacock, C.L., Ostle, N.J., Griffiths, R., Chapman, P.J., Holden, J., Banwart, S., McNamara, N.P., Whitaker, J. (2025). Measurements of respiration, incorporation of litter carbon into soil fractions, and microbial community data using mineral-amended soils. NERC EDS Environmental Information Data Centre 10.5285/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 This dataset includes measurements made during a laboratory-based study conducted between 10/2020 – 03/2021 on mineral amended soils with additions of two 13C labelled plant litters of contrasting quality. Data collected include: soil respiration (Carbon Dioxide (CO2))¬, total C content of bulk and soil fractions including mass and C recovery as a measure of the efficacy of the fractionation method, 13C enrichment of CO2 and soil fractions expressed as litter derived C, microbial data from bacterial (16S Small subunit ribosomal RNA) and fungal (internal transcribed spacer region 2) high throughput sequences. These data were collected to determine the relative effects of litter quality and soil mineralogy on the formation of soil C from plant material and may be useful to link plant inputs, soil microbes and soil mineralogy in the next generation of SOC models. Resolving the key fundamental mechanisms that control SOM persistence is crucial to optimise land management for SOC and predict soil C feedback to climate change. The experiment was part of the NERC project NE/S005137/2 Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 Elias, D.M.O. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0000-0002-2674-9285 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Elias, D.M.O. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0000-0002-2674-9285 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information pointOfContact Mason, K.E. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0000-0003-3426-3178 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Goodall, T. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0000-0002-1526-4071 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Taylor, A. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0009-0002-1241-2013 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Zhao, P. Université Catholique de Louvain pengzhi.zhao@uclouvain.be http://orcid.org/0000-0001-6675-7338 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Farina, A.O. Universidade de Santiago de Compostela a.otero.farina@gmail.com http://orcid.org/0000-0002-0053-9482 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Chen, H. Lancaster University h.chen18@lancaster.ac.uk http://orcid.org/0000-0001-8472-6113 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Peacock, C.L. University of Leeds c.l.peacock@leeds.ac.uk http://orcid.org/0000-0003-3754-9294 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Ostle, N.J. Lancaster University n.ostle@lancaster.ac.uk http://orcid.org/0000-0003-3263-3702 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Griffiths, R. Bangor University robert.griffiths@bangor.ac.uk http://orcid.org/0000-0002-3341-4547 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Chapman, P.J. University of Leeds p.j.chapman@leeds.ac.uk http://orcid.org/0000-0003-0438-6855 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Holden, J. University of Leeds j.holden@leeds.ac.uk http://orcid.org/0000-0002-1108-4831 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Banwart, S. University of Leeds EMAIL NOT PROVIDED http://orcid.org/0000-0001-7223-6678 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author McNamara, N.P. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0000-0002-5143-5819 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Whitaker, J. UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk http://orcid.org/0000-0001-8824-471X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC EDS Environmental Information Data Centre info@eidc.ac.uk publisher UK Centre for Ecology & Hydrology enquiries@ceh.ac.uk owner soil mineralogy litter GEMET - Concepts, version 4.1.3 2019-01-28 creation Biodiversity Carbon stable isotope tracers Microbial communities otherRestrictions no limitations otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Elias, D.M.O., Mason, K.E., Goodall, T., Taylor, A., Zhao, P., Farina, A.O., Chen, H., Peacock, C.L., Ostle, N.J., Griffiths, R., Chapman, P.J., Holden, J., Banwart, S., McNamara, N.P., Whitaker, J. (2025). Measurements of respiration, incorporation of litter carbon into soil fractions, and microbial community data using mineral-amended soils. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 English utf8 biota Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/fddf7d9d-9694-4167-bbfe-8fb9d49f02a7.zip Supporting information Supporting information available to assist in re-use of this dataset information nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Soil was collected from a commercial farm near Glassonby, Penrith and amended with pristine minerals (Kaolinite, Montmorillonite or Goethite) to create soil mineral treatments. Two 13C labelled litters were prepared in-house and added to soils to investigate the effect of litter quality and soil mineralogy on soil organic matter formation. The laboratory experiment was carried out at UK Centre for Ecology & Hydrology in Lancaster and soils were incubated under controlled conditions for 126 days. Respiration of litter-C was quantified by headspace CO2 and 13CO2 analysis on microcosms throughout the 126-day duration of the experiment and application of a two-component isotope mixing model. Concentrations of CO2 were analysed on a calibrated PerkinElmer Autosystem XL Gas Chromatograph. δ13C values of CO2 were analysed using a Picarro cavity ring-down spectrometer (model: G2201-I, Picarro, Inc. CA, USA). For enriched samples the instrument was calibrated for δ13C-CO2 using in-house prepared gas standards of 97.7 ‰ and 815 ‰. Litter carbon in soil fractions was determined by physicochemical density soil fractionation, and fractions analysed for C and δ13C using a Costech ECS-4010 Elemental Analyser (Costech Analytical Technologies, USA) coupled to a calibrated (cane sugar (−11.64 ‰), beet sugar (−26.03 ‰), enriched glucose (197.90 ‰) (IsoAnalytical, UK)) CRDS Picarro G-2131i isotopic analyser (Picarro Inc. CA, USA) and application of a two-component isotope mixing model. Bacterial and fungal community compositions were assessed using 16S rRNA and ITS2 amplicon sequencing using the Illumina MiSeq platform with V2 500 cycle chemistry. Sequences were processed using the DADA2 pipeline to trim, quality-filter, de-noise, de-replicate, generate amplicon sequence variant tables and assign taxonomies.