BGS Hosted Metadata
Phenotypic traits of Rhizobium leguminosarum TRX19 clones evolved in the presence of different lytic bacteriophages
This dataset comprises phenotypic data of Rhizobium leguminosarum TRX19 clones evolved in the presence or absence of lytic phages. The clones assayed were isolated from populations of R. leguminosarum which had been evolved in lab media for twenty 73hr 'transfers'. Clones were assessed for their resistance to the ancestral phages (reduction in bacterial growth), their ability to form symbioses (plant biomass, nitrogen content and nodule counts) and traits associated with colonisation (biofilm production, swimming and swarming motility and gelatinase activity). This experiment tests the hypothesis that evolution for phage resistance will drive the loss of symbiosis functions in rhizobia due to antagonistic pleiotropy.
Simple
- Date (Publication)
- 2026-03-04
- Date (Creation)
- 2025-09-04
- Citation identifier
- https://catalogue.ceh.ac.uk/id/12f2f235-8e18-40c7-9113-acb85de9edd7
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Sheffield
Ellie Harrison
Point of contact University of Sheffield Pain, C.
Author University of Sheffield Harrison, E. Author University of Sheffield EMAIL NOT PROVIDED
Owner NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher
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GEMET - Concepts, version 4.1.3
- Keywords
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- Evolutionary ecology
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Bacteriophage
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Rhizobia
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Antagonistic coevolution
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Language
- English
- Character set
- UTF8
- Topic category
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- Environment
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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This dataset contains assay data from an evolution experiment assessing the impact of evolution with different lytic phages on the symbiotic performance of rhizobia. Briefly, strain Rhizobium leguminosarum TRX19 was evolved in lab media with 1 of 3 phages or in the absence of phage. Clones were isolated from evolved populations and screened for resistance, symbiotic function and phenotypes related to colonisation. Most of the data are raw values acquired from assays of the evolved clones. These include: growth with host plant - collecting plant biomass, nodule counts and nitrogen content via Isotope ratio mass spectrometry. Phenotypic analysis - biofilm production, swimming and swarming motility, growth rate in liquid broth and gelatinase assays Phage resistance data is presented as RBG - reduction in bacterial growth - generated as the ratio of the density (OD600) of cultures grown in a microtitre plate either with or without phages. The ratio of phage exposed to phage free bacterial growth indicates the severity of the infection.
Metadata
- File identifier
- 12f2f235-8e18-40c7-9113-acb85de9edd7 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2026-03-04T09:03:49
- Metadata standard name
- UK GEMINI
- Metadata standard version
-
2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
