Date (Creation)

2020-05-27

Date (Revision)

2020-05-27

Date (Publication)

2020-05-27

Date (released)

2020-05-27

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/1cf37889-9f77-41b9-8c49-b175fbd03406

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01333

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Newsham, K., Misiak, M., & Worland, M. (2020). Pseudogymnoascus roseus in soil at Mars Oasis, Alexander Island, under different temperature, irrigation and substrate treatments (Version 1.0) [Data set]. UK Polar Data Centre, Natural Environment Research Council, UK Research & Innovation. https://doi.org/10.5285/1cf37889-9f77-41b9-8c49-b175fbd03406

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Newsham, Kevin K.		Author
British Antarctic Survey	Misiak, Marta		Author
British Antarctic Survey	Worland, M. Roger		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Fungi

Theme

• Mars Oasis

• Pseudogymnoascus roseus

• enzyme activity

• quantitative-PCR

• snow depth

• soil decomposer fungus

• soil warming

• soil water potential

• surface soil temperature

Place

• Alexander Island, Mars Oasis Antarctica

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC data policy and supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

We strongly encourage contacting Kevin Newsham ( kne@bas.ac.uk) for more information regarding this dataset, including guidance on its reuse.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2007-12-01

End date

2018-12-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

The field experiment was set up in December 2007, at Mars Oasis. 64 circular plots (1 m diameter) were established, half of which were covered with open top chambers. In December 2007, 2009, 2010, 2011 and 2012, each of the 64 plots was assigned to one of four groups (16 plots in each group) and the soil underwent four different treatments: unamended; received glucose; received glycine (amino acid); received powdered tryptic soy broth (mix of amino acids and protein). Half of the chambered and half of the unchambered plots were then raised to 100% water holding capacity. The experimental layout resulted in 16 open top chamber-irrigation-substrate treatment combinations, each replicated four times in a randomised design.

Temperature was monitored in three chambered and three unchambered plots between December 2009 and December 2012. Frozen soil samples were returned to the UK, where water potentials were measured. Laboratory experiments were also undertaken. Three Pseudogymnoascus roseus isolates were obtained from Mars Oasis soil using the soil plate method and identified by sequencing of ribosomal DNA. Quantitative-PCR assays were used to estimate the concentration of Pseudogymnoascus DNA present in soil. The hyphal extension rate analyses were undertaken in the lab, where isolates were grown for 10 weeks on soil extract medium at temperatures cycling daily from 2-18 ° C, 2-21 ° C and 2-24 ° C and at water potentials of -1.06 MPa, -3.60 MPa, -6.00 MPa and -10.80 MPa. At the end of the growth rate experiments, extracellular enzymes were extracted from colonies and the activities of β -1,4-glucosidase (cellulase), N-acetyl- β -glucosaminidase (chitinase), leucine aminopeptidase and alkaline and acid phosphatases were measured using p-nitrophenyl substrates. The impact of temperature cycle and water potential treatments on numbers of P. roseus conidia produced per colony was measured. The treatment effects on conidial germination were also quantified at modified water potentials.

Complete methods can be downloaded with the data via the Get Data link.

Data collection:

Soil temperatures: TinyTag Plus 2 TGP-4017, Gemini Data Loggers Ltd., Chichester, UK

Soil water potentials: WP4C, Decagon Devices, Inc., Pullman, WA, USA

Q-PCR thermocycler: Eco 48 machine, PCRMax Ltd., Witford, UK

Image analysis: ImageJ, National Institute of Health, Bethesda, MD, USA

Plant growth cabinets: MCR-350 Versatile Environmental Test Chamber, Sanyo, Osaka, Japan

Plate reader: Sunrise, TECAN, Austria

Data quality:

Raw data are shown. The data have not been cleaned. Missing data are indicated by NA. There are 1-2 missing data points in the water potential, DNA concentration, conidial density and hyphal extension rate data, caused principally by lack of material or analysis failure. 22 values are missing from the specific enzyme activity data, all of which were caused by analysis failure.

Metadata

File identifier

1cf37889-9f77-41b9-8c49-b175fbd03406 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2020-05-27

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact