BGS Hosted Metadata
Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016)
The data set includes the results of a laboratory analysis in 2016, investigating the presence of trypanosomes and prevalence of tsetse endosymbionts in tsetse flies. The tsetse flies were sampled in Hurungwe District, Mashonaland West Province, Zimbabwe, from February 2014 to November 2014. Flies were sampled using a combination of Epsilon traps and fly rounds, both established techniques for sampling tsetse. Tsetse were stored prior to laboratory analysis using Polymerase chain reaction (PCR) techniques in 2016. The data include two species of tsetse, Glossina pallidipes and Glossina morsitans morsitans. Trypanosome species investigated include Trypanosoma brucei s.l., T. b. rhodesiense, T. vivax, T. congolense, T. simiae, T.simiae (Tsavo) and T. godfreyi. Endosymbionts included in the study were Sodalis glossinidius and Wolbachia spp. Hurungwe District is the only sleeping sickness focus in Zimbabwe and an increase in cases had been detected in years preceding this study. The objective of the study was to investigate the trypanosome species present in the tsetse population and their association with tsetse endosymbionts. This study was conducted as part of research into the relationship between trypanosomiasis, well-being and ecosystems by the Dynamic Drivers of Disease in Africa Consortium (DDDAC). The research was funded by NERC with support from the Ecosystem Services for Poverty Alleviation Programme (ESPA). Full details about this dataset can be found at https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
Simple
- Date (Publication)
- 2017-04-21
- Date (Creation)
- 2016-04-19
- Citation identifier
- https://catalogue.ceh.ac.uk/id/1e435dd3-ed93-4961-a136-ffa477731e1c
- Citation identifier
- doi: / 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Other citation details
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Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Point of contact
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Organisation name Individual name Electronic mail address Role The Royal (Dick) School of Veterinary Studies, University of Edinburgh
Anderson, N.
Point of contact University of Edinburgh Goodwin, M. Author University of Edinburgh Webber, F. Author The University of Edinburgh
Hamill, L.C. Author The University of Edinburgh
Anderson, N.E. Author Department of Livestock and Veterinary Services, Zimbabwe
Shereni, W. Author Department of Livestock and Veterinary Services, Zimbabwe
Nyakupinda, L. Author Central Veterinary Research and Diagnostic Laboratory, Zimbabwe
Gwenhure, L.F. Author The University of Edinburgh Macleod, E. Author NERC Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian
- Maintenance and update frequency
- Not planned
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GeoNames
- Keywords
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Dynamic Drivers of Disease in Africa Consortium (DDDAC)
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Sleeping sickness
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- Other restrictions
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- no limitations
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© The University of Edinburgh
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© Central Veterinary Research and Diagnostic Laboratory, Zimbabwe
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© Department of Livestock and Veterinary Services, Zimbabwe
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If you reuse this data, you should cite: Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Health
- Begin date
- 2014-02-01
- End date
- 2016-04-12
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- Unique resource identifier
- WGS 84
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/1e435dd3-ed93-4961-a136-ffa477731e1c Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/1e435dd3-ed93-4961-a136-ffa477731e1c.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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The data presents the molecular analysis of 101 Glossina morsitans morsitans and 108 Glossina pallidipes tsetse sampled in Hurungwe District, Mashonaland West Province, Zimbabwe. Tsetse were sampled using Epsilon traps and fly rounds as described by Shereni et al. (2016). Tsetse were stored in cryotubes with desiccant beads at room temperature. DNA was extracted from the tsetse using Qiagen DNeasy kits and then subjected to polymerase chain reactions (PCR) to detect the primary endosymbiont, Wigglesworthia glossindia. Samples from which no DNA was amplified were discounted from further molecular analysis. All remaining samples were subjected to PCR reactions to detect African trypanosomes and the secondary tsetse symbionts, Sodalis glossinidius and Wolbachia. All PCRs included a negative and a positive control. The data were collated in an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.
Metadata
- File identifier
- 1e435dd3-ed93-4961-a136-ffa477731e1c XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-11-13T16:21:15
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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