Date (Publication)

2022-06-27

Citation identifier

https://catalogue.ceh.ac.uk/id/34df30f2-3163-4c11-8743-3732e49220fb

Citation identifier

doi: / 10.5285/34df30f2-3163-4c11-8743-3732e49220fb

Other citation details

Pattison, Z., Quilliam, R.S., Oliver, D., Willby, N.J. (2022). High spatial resolution seasonal distributions of faecally-derived waterborne and sediment bacteria in standing waters, Glasgow, UK, 2016-2017. NERC EDS Environmental Information Data Centre 10.5285/34df30f2-3163-4c11-8743-3732e49220fb

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of Stirling	Willby, N.J.	n.j.willby@stir.ac.uk	Point of contact
University of Newcastle	Pattison, Z.	Zarah.pattison@newcastle.ac.uk	Author
University of Stirling	Quilliam, R.S.	richard.quilliam@stir.ac.uk	Author
University of Stirling	Oliver, D.	david.oliver@stir.ac.uk	Author
University of Stirling	Willby, N.J.	n.j.willby@stir.ac.uk	Author
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Custodian
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Publisher
University of Stirling		n.j.willby@stir.ac.uk	Owner

GEMET - INSPIRE themes, version 1.0

• Human Health and Safety

• Environmental Monitoring Facilities

GEMET - Concepts, version 4.1.3

• microbiology
• urban area

Keywords

• Water quality

• Glasgow

• faecal indicators

• spatial variation

• connectivity

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

Other restrictions

Other constraints

This resource is available under the terms of the Open Government Licence

Use constraints

Other restrictions

Other constraints

If you reuse this data, you should cite: Pattison, Z., Quilliam, R.S., Oliver, D., Willby, N.J. (2022). High spatial resolution seasonal distributions of faecally-derived waterborne and sediment bacteria in standing waters, Glasgow, UK, 2016-2017. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/34df30f2-3163-4c11-8743-3732e49220fb

Spatial representation type

Text, table

Distance

1  urn:ogc:def:uom:EPSG::9001

Language

English

Character set

UTF8

Topic category

• Biota
• Health

Begin date

2016-01-01

End date

2017-02-28

Unique resource identifier

OSGB 1936 / British National Grid

Distribution format

Name	Version
Comma-separated values (CSV)

Distributor contact

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Distributor

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/data/34df30f2-3163-4c11-8743-3732e49220fb	Download the data

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/sd/34df30f2-3163-4c11-8743-3732e49220fb.zip	Supporting information

Hierarchy level

Dataset

Other

dataset

Statement

Sub-surface water samples and cores of the upper 5cm of sediment obtained from multiple geolocated points within each of 15 water bodies via boat-based sampling. For consistency all sites were sampled 3 hours after sunrise in summer (July) and on ice-free days in winter (Dec/Jan). Sampling intensity was dependent on water body size. Bird distribution and numbers were recorded for an hour from a suitable vantage point at the same time of sampling. A separate water sample was collected from a mid-point in each site for laboratory analysis of metals, nutrients and other standard determinands (Thermo iCap 6000 Series or Dionex DX-120). Dissolved oxygen, pH, electrical conductivity and temperature were measured in the field using a HACH multiprobe. Standard water body characteristics (area, elevation, perimeter etc) are sourced from the U.K. Lakes Portal ( https://uklakes.ceh.ac.uk/) Water samples for microbial analysis were stored at 4 C and processed within 6 h of collection. 100ml samples at three dilutions were filtered through 0.45-m membrane filters, placed onto the surface of Membrane Lactose Glucoronide Agar (MLGA) agar. Plates were incubated at 37 deg C and counted after 24 h. Counts are reported as colony forming unit (CFU) per 100 ml water. A mixture of sediment (~2.5 g) and 50ml Phosphate buffered saline (PBS) was used to enumerate E. coli in the sediment. All sediment dilutions were made by placing samples in sterile PBS and shaking them. Each dilution was spread using disposable sterile loops onto MLGA agar plates and incubated at 37 deg C for 24 hours before counting colonies. To quantify abundance of Campylobacter, I. Enterococci, E. coli 0157 and E.coli in sediment a single composite 1g subsample was placed into a sterilised glass vial of 100ml LB broth. A 100ug sample of the aliquot of each sample was spread over the surface of agar plates with a disposable sterile loop and incubated following isolation and incubation protocol for each type of bacteria. Colonies were counted, and results are reported as CFU per g oven-dried soil. Data were added to an Excel spreadsheet and exported as a .csv file for deposit into the EIDC.

Metadata

File identifier

34df30f2-3163-4c11-8743-3732e49220fb XML

Metadata language

English

Character set

8859 Part 1

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2026-01-30T08:45:47

Metadata standard name

UK GEMINI

Metadata standard version

2.3

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Point of contact