Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature

This dataset contains information on how variations on temperature and photoperiod affects poplar seedling growth in Eco chambers. Plants were propagated on sterile media (1/2MS, 0.7% Agar, 1mg/L IAA) by cutting the shoot tip and 3-4 leaves and placing them in a weck jar on new medium. Once the explants rooted after incubation at 200 mmol light, 21C day/18C night, long day conditions (16:8) and ambient humidity, they were transplanted onto soil (6:2:1 Peat-free: sand-vermiculite, fertilizer: 4 g/L Osmocote exact standard 15-9-12) and grown first under protective covers and then open in 24C, long day conditions (LD), 65% humidity, 350mmol light (experiments R1-3) or transferred into the Eco chamber (temperature as indicated in experiment, 80-98% humidity, 350mmol LED light, long day conditions). After 3-4 weeks, plants of R1-R3 were transferred in the conditions described in the experiment (R1: 24C SD vs 15C LD; R2: 15C SD vs 15C LD; R3: 15C LD vs 24C LD) and grown for a month before moving them into 5C low light conditions which resulted in senescence and leaf drop. Eco chamber plants were grown in the Eco chamber for about four weeks and after not showing senescence, harvested directly after the experiment without cold incubation. All plants were measured in height and diameter (6cm above soil), leaf number and area regularly and gas exchanged was measured with a TARGAS-1 instrument (Hansatech) at 500mmol light intensity. After the experiment, cross-sections were taken and embedded in resin for further microscopic analysis (data not ready and not included). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898

Date (Publication): 2025-04-17

Citation identifier: https://catalogue.ceh.ac.uk/id/46c23be6-63ba-400d-859b-050dcb207898

Citation identifier: doi: / 10.5285/46c23be6-63ba-400d-859b-050dcb207898

Other citation details: Hellmann, E., Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre 10.5285/46c23be6-63ba-400d-859b-050dcb207898

Point of contact

Organisation name	Individual name	Electronic mail address	Role
Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde	Hellmann, E.	Eva.hellmann@slcu.cam.ac.uk	Point of contact
Department of Geography, Cambridge, The Sainsbury Laboratory, Cambrigde	Hellmann, E.	Eva.hellmann@slcu.cam.ac.uk	Author
Department of Geography, Cambridge	Friend, A.D.	Adf10@cam.ac.uk	Author
University of Cambridge		EMAIL NOT PROVIDED	Owner
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Custodian
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Publisher

GEMET - INSPIRE themes, version 1.0

Habitats and Biotopes

GEMET - Concepts, version 4.1.3

Keywords

Poplar
day-length
growth

Access constraints: Other restrictions

Other constraints: no limitations

Use constraints: Other restrictions

Other constraints: This resource is available under the terms of the Open Government Licence

Use constraints: Other restrictions

Other constraints: If you reuse this data, you should cite: Hellmann, E., Friend, A.D. (2025). Growth of poplar seedlings grown in chambers following variations in photoperiod and temperature. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/46c23be6-63ba-400d-859b-050dcb207898

Language: English

Character set: UTF8

Topic category

Biota

Begin date: 2023-01-01

End date: 2023-12-31

Distribution format

Name	Version
Comma-separated values (CSV)

Distributor contact

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Distributor

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/data/46c23be6-63ba-400d-859b-050dcb207898	Download the data

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/sd/46c23be6-63ba-400d-859b-050dcb207898.zip	Supporting information

Hierarchy level: Non geographic dataset

Other: nonGeographicDataset

Conformance result

Title: Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication): 2010-12-08

Statement: The data have been generated using calipers, a laser distance meter, Image J for leaf area and a TARGAS-1 instrument for photosynthetic gas exchange. The data have been averaged between 20-25 plants and the standard deviation calculated.

Metadata

File identifier: 46c23be6-63ba-400d-859b-050dcb207898 XML

Metadata language: English

Character set: 8859 Part 1

Hierarchy level: Non geographic dataset

Hierarchy level name: nonGeographicDataset

Date stamp: 2026-01-05T08:38:21

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Point of contact

Overviews

Provided by

Access to the catalogue

Read here the full details and access to the data.

Simple

Conformance result

Metadata

Overviews

Keywords

Provided by

Share on social sites