Date (Creation)

2021-01-26

Date (Revision)

2021-01-26

Date (Publication)

2021-01-26

Date (released)

2021-01-26

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/4d781314-b2a6-47fe-aab9-4364a9022e44

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01461

Codespace

https://data.bas.ac.uk/

Unique resource identifier

NE/P006213/1

Codespace

award

Other citation details

Please cite this item as: Mitchell, E., & Pond, D. (2024). Phytoplankton enumeration and biomass calculations from samples collected in the Fram Strait, Greenland Basin and Svalbard region during 2017-2018 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/4d781314-b2a6-47fe-aab9-4364a9022e44

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
Scottish Association For Marine Science	Mitchell, Elaine		Author
University of Stirling	Pond, David		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Ecological Dynamics > Biomass
• EARTH SCIENCE > Biosphere > Microbiota Taxonomy > Phytoplankton
• EARTH SCIENCE > Oceans > Marine Environment Monitoring

Theme

• C:N ratio

• Fram Strait

• biomass

• enumeration

• phytoplankton

Place

• Greenland Basin, Norsk Trough, Fram Strait, Arctic

• Kongsfjord Basin Arctic

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes
• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC data policy and supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

None.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Larger work citation

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Environment
• Oceans

Begin date

2018-05-10

End date

2018-06-08

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

2 cruises: JR17005 / JR18007

Seawater was collected from two depths from a standard environmental CTD cast as close to net collections. Sampling depths were selected based on the PAR irradiance readings from the CTD at the surface of the water (approx. 2m) after being initially stabilised at 10m and bought back to the surface. For phytoplankton samples two depths were selected - Surface and the Chlorophyll maximum. 10L acid washed carboys and acid washed tubing were used to collect the water samples. The carboys were stored in either the cold room or on deck in a low light area. Location of the collected water for storage until processing was dependent on the temperature of the surface water at the point of collection. 4ml of Lugol's solution, 1% (final concentration) was dispensed into 500ml glass amber bottles. 400ml of water sample was added, and samples stored in the cold room at 4-5°C.

Laboratory analysis:

Before observation through a light microscope, samples fixed with 1% Lugol's iodine were settled using a Hydro-Bios glass plate chamber and 50ml tower. The inverted samples were poured into the tower, so it was just over full. A glass coverslip was placed over the top of the tower, ensuring there were no bubbles in the column. Vacuum pressure holds the chamber set up together. The base plate and tower were then carefully lifted into a tray and left to settle for a minimum of 20 hours.

After adequate settling time, the chamber was removed from the tray and distilled water was dropped either side of the tower base. The tower was removed by gently pushing it across the base plate, followed by a square coverslip, ensuring there was no gap to minimise bubbles. The baseplate and tower were then lifted carefully, and the remaining contents were emptied into a waste beaker. The base plate was dried, ready for microscopy. The waste beaker, tower and tower cover slip were washed in a sink with hot water.

The prepared base plates were transferred to an inverted light microscope, the light source was set to a preferred intensity and viewed using a x20 objective, Phase2 contrast conditions.

Where cells were in high abundance, a minimum of 100 cells were counted for statistical reasons, which was achieved by determining a set number of fields of view. Where cells are rare or in low abundance, the whole of the chamber was counted.

A calibrated eyepiece graticule was used to size phytoplankton cells from the chlorophyll maximum samples only. Imaging software confirmed eyepiece sizing on a select number of cells. Conversion of cell sizes to biomass volumes was achieved using geometric calculations - specific for each cell type. Calculations take into consideration the shrinkage effect of the fixative on the cells to give a live volume. From this live volume C:N ratios were calculated.

Cell enumeration results for each species at each station are provided as - cell numbers/litre.

Cell sizes for biomass calculations are provided as - Length in µm, Breadth in µm.

Biomass volumes are provided as - pgC per cell and Total µgC for each station.

Data collection:

Standard CTD and associated software used to determine ocean parameters and define collection depths on board the ship.

Sample analysis conducted on a Zeiss S100 inverted microscope by Elaine Mitchell. Axio Imager software version 4.1 was used to take images and confirm eyepiece graticule sizing.

Data from microscope analysis was entered into Comma Separated Value (csv) files for cell enumerations, biomass and C:N ratio calculations.

Data quality:

Sizing of cells for biomass calculations were done using an eye piece graticule on the microscope, a maximum of 30 cells of each type were sized for each sample - where possible - from the chlorophyll maximum depth. A small number of images were taken and the manual eyepiece measurements were compared to the measurements taken using the Axio imager software and in each case they were almost the same if you rounded up the imaging sizes to the nearest micron.

David Pond at Stirling University checked the data for errors/alterations before submission.

Metadata

File identifier

4d781314-b2a6-47fe-aab9-4364a9022e44 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2021-01-26

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact