Date (Creation)

2021-06-30

Date (Revision)

2021-06-30

Date (Publication)

2021-06-30

Date (released)

2021-06-30

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/53737fbe-8cb8-4e3d-bc48-2b21e7c9d543

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01516

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Fraser, K. (2021). Harpagifer antarcticus and Lipophrys pholis protein metabolism (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/53737fbe-8cb8-4e3d-bc48-2b21e7c9d543

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of Plymouth	Fraser, Keiron		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Ecological Dynamics > Adaptation
• EARTH SCIENCE > Oceans > Marine Biology > Fish
• EARTH SCIENCE > Oceans > Marine Environment Monitoring

Theme

• Protein metabolism

• RNA

• fish growth

• polar fishes

• protein synthesis

Place

• Antarctic Peninsula, Adelaide Island, Rothera Point Antarctica

• Weymouth United Kingdom

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes
• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Data is released under Open Government Licence V3.0:

Use constraints

Other restrictions

Other constraints

No restrictions apply.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

doi

Codespace

doi

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

doi

Codespace

doi

Association Type

Cross reference

Unique resource identifier

doi

Codespace

doi

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Environment
• Oceans

Begin date

2004-01-01

End date

2004-12-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Collection and husbandry of fish

Harpagifer antarcticus were collected by hand from sublittoral sites at Rothera Point, Adelaide Island, Antarctic Peninsula (67.56861 S, 68.125 W) by SCUBA divers and held in a through-flow aquarium until return to the UK in a refrigerated transport system. In the UK, fish were maintained in a recirculating flow aquarium, under an automatic 12h light : dark regime (water temperature 0°C ± 1.0°C, salinity 34 - 36 PSU). The fish were fed twice weekly to satiation on shelled krill (Euphausia superba). Lipophrys pholis were collected subtidally in Weymouth (UK) by a commercial supplier and fed on chopped white fish (New Zealand Hoki, Macruronus novaezelandiae) as they refused krill. The mean water temperature of the L. pholis stock tank was 14°C ± 1.0°C. With the exception of temperature and diet, husbandry conditions for both species were identical.

Temperature acclimation

Experimental animals were weighed in water (± 0.1g) and digitally photographed to allow individual identification. The fish were placed in pairs, in jacketed aquaria (18.8 x 20.3 x 22.0cm) containing clean, aerated seawater at their stock tank temperature. Water temperatures were maintained (± 0.1°C) using a thermocirculator (Grant Instruments, Cambridge, UK). Once daily the aquaria were divided in half using a perspex insert, thereby allowing fish to be fed individually to satiation and their food consumption measured (± 0.1g).

Adjustment of aquaria to the required experimental water temperatures (H. antarcticus, -1, 1 and 3 °C, L. pholis, 3, 8, 13 and 18 °C) was carried out at a rate of 0.1°C hour-1 (H. antarcticus) or 0.5°C hour-1 (L. pholis), up to a maximum of 0.8°C day-1 (H. antarcticus) or 4°C day-1 (L. pholis). Fish were acclimated to the experimental temperature for 28 days prior to the measurement of protein synthesis, as Antarctic fish can take 3-4 weeks to acclimate to 4 °C and are the slower of the two species to acclimate. The fish were weighed on the first and penultimate day of the acclimation. Specific growth rates (SGR) were calculated for each fish.

equation (1)

SGR = (ln(W2)-ln(W1)/t) x 100

where SGR is expressed as % body mass. day-1, W1 and W2 represent the mass at the start and end of the acclimatization period respectively and t is the time in days between mass measurements.

Protein synthesis measurement

Whole animal protein synthesis rates were measured using a modification of the flooding dose method. Groups of fish were injected in the peritoneum with a flooding dose of unlabelled and 3H labelled phenylalanine (10microl.g-1 fish wet mass of 135mmol.l-1 L-[2,6-3H] phenylalanine at 3.6MBq.ml-1 (GE Healthcare, Little Chalfont, UK)). Fish were killed and frozen in liquid nitrogen after 1, 2 and 4h to allow validation of the flooding dose time-course (H. antarcticus, 3°C; L. pholis, 3 and 18°C). In other experimental treatments fish were killed after 2h, H. antarcticus, -1 and 1°C; L. pholis, 8 and 13°C. Baseline, pre-injection phenylalanine concentrations were measured in both species (n=10) to allow the calculation of phenylalanine flooding levels. All samples were frozen in liquid nitrogen and stored at -80°C prior to analysis.

Sample analysis

Samples were analysed following Fraser et al., (2002). The frozen fish were homogenized in a known volume of ice-cold, 0.2M perchloric acid (PCA). The homogenate was mixed thoroughly and a 2ml aliquot removed for analysis. The aliquot was centrifuged (Eppendorf 5810R, swing bucket rotor, 3980 x g, 10 min, 4°C) to separate the protein precipitate from the intracellular free-pool. The supernatant, was decanted and the NaOH soluble protein in the pellet measured using bovine serum albumin (Sigma-Aldrich, Poole, UK) as the standard. Subsequently, the pellet was ...(34)

Data collection:

Thermocirculator - Grant Instruments Cambridge

Centrifuge - Eppendorf 5810R, swing bucket rotor

Rotary evaporator - Buchi

Scintillation counter - Wallac 1409 LSC

Data quality:

Data did not require cleaning and no data was lost.

Metadata

File identifier

53737fbe-8cb8-4e3d-bc48-2b21e7c9d543 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2021-06-30

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact