BGS Hosted Metadata
Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD)
This dataset contains information about the development of a paper analytical device for the detection of the microcystin toxin. Water samples were collected weekly from Rostherne Mere and Tatton Park Lake, Cheshire between 20/07/2022 and 12/10/2022. Samples were analysed for the presence of microcystin-producing genes and released microcystin. A paper analytical device incorporating a protein phosphatase inhibition assay was also used to monitor microcystin levels. Preliminary user evaluation of the paper analytical devices and associated mobile photo applications is also provided. The work was supported by the Natural Environment Research Council (Grant NE/X011607/1). Full details about this dataset can be found at https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
Simple
- Date (Publication)
- 2024-06-12
- Citation identifier
- https://catalogue.ceh.ac.uk/id/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- Citation identifier
- doi: / 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- Other citation details
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Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- Point of contact
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Organisation name Individual name Electronic mail address Role Manchester Metropolitan University
Hill, M.
Author Manchester Metropolitan University
Jenkinson, L.A.
Author Manchester Metropolitan University
Dean, A.P. Author Manchester Metropolitan University
Redfern, J. Author Manchester Metropolitan University
Shaw, K.J. Author Manchester Metropolitan University
Shaw, K.
Point of contact NERC EDS Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian Manchester Metropolitan University EMAIL NOT PROVIDED
Owner
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GEMET - INSPIRE themes, version 1.0
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Environmental Monitoring Facilities
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- Keywords
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- Environmental risk
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Cheshire Meres
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Water chemistry
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Cyanobacteria
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Algal blooms
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Microfluidics
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Public engagement
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Lab on a chip
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- Spatial representation type
- Text, table
- Distance
- 1000 urn:ogc:def:uom:EPSG::9001
- Language
- English
- Character set
- UTF8
- Topic category
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- Environment
- Begin date
- 2022-06-20
- End date
- 2022-10-12
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- Unique resource identifier
- OSGB 1936 / British National Grid
- Distribution format
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Name Version Comma-separated values (CSV)
jpeg
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/5fe25b28-f10b-467b-8812-0b1ee6fd7491 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/5fe25b28-f10b-467b-8812-0b1ee6fd7491.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Water samples were taken on a weekly basis from three sampling locations at each site. Samples were filtered through a 0.45 µm filter and DNA was extracted from the filter paper using a Qiagen PowerWater kit before being analysed using real-time PCR for detection of the mycE gene, alongside no template controls. Water samples were analysed using an ENZO Life Sciences Microcystins (Adda specific) ELISA kit, alongside microcystin reference standards and controls. For the development of the paper analytical device, protein phosphatase 1a was produced using protein expression and confirmed using spectrophotometry. Efficiency was evaluated by comparison to commercially available products and comparing absorbance values for the conversion of pNPP. The paper analytical device incorporating the protein phosphatase inhibition assay was then used to detect microcystin. Colourimetric change was recorded for samples and controls using Image J. Preliminary user evaluation of the paper analytical device was obtained using qualitative feedback.
Metadata
- File identifier
- 5fe25b28-f10b-467b-8812-0b1ee6fd7491 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-11-13T16:22:33
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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