Date (Creation)

2024-03-11

Date (Revision)

2024-03-11

Date (Publication)

2024-03-11

Date (released)

2024-03-11

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/60b777b4-0bd6-48c3-a301-c700854fbfa1

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01825

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Morley, S.A., Bates, A.E., Clark, M.S., Fitzcharles, E., Smith, R., Stainthorp, R.E., & Peck, L.S. (2024). Acclimation and acclimatisation of marine ectotherms collected at Rothera Research Station and Scott Base in Antarctica between 2004 and 2015 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/60b777b4-0bd6-48c3-a301-c700854fbfa1

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Morley, Simon A		Author
University of Victoria	Bates, Amanda E		Author
British Antarctic Survey	Clark, Melody S		Author
British Antarctic Survey	Fitzcharles, Elaine		Author
British Antarctic Survey	Smith, Rebecca		Author
National Oceanography Centre	Stainthorp, Rose E		Author
British Antarctic Survey	Peck, Lloyd S		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Ecological Dynamics > Adaptation
• EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
• EARTH SCIENCE > Oceans > Marine Environment Monitoring

Theme

• Antarctic

• Southern Ocean

• acclimation

• acclimatisation

• climate change

• physiology

• resilience

• warming

Place

• Ryder Bay, Rothera Research Station Antarctica

• Ross Island, Scott Base Antarctica

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes
• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC data policy and supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

None.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Environment
• Oceans

Begin date

2004-01-01

End date

2015-12-31

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Experiments were conducted between 2006 and 2015 with individuals hand collected by SCUBA divers in the austral summer, from 6 - 15m depth, near Rothera Research Station, Adelaide Island (67° 34' 2'' S, 68° 08' 0'' W). Common marine ectotherms were selected from different phyla to represent a range of functional groups, for which aquarium husbandry is well established and long term temperature limits have been estimated under normoxia.

All animals remained submerged throughout the transfer from the sea to the flow-through aquarium system at the station. To control for size-dependent effects on survival we selected individuals of a similar size within each species group at the start of the experiment, and only studied fully reproductive adults.

The 3.0 °C temperature-oxygen experiment was conducted in flow through aquaria at Rothera Research Station. In Rothera, the tanks had a constant exchange of seawater that was balanced to allow temperature and oxygen treatments to be maintained while preventing any build-up of metabolic waste. In all experiments, seawater chemistry was monitored every 2-3 days using Nutrafin aquarium test kits. Ammonia, nitrite and nitrates were maintained well below 0.4, 0.2 and 5 mg L-1 to prevent toxicity from metabolic by-products.

A small piece of one tentacle was removed from each anemone and preserved in 96% ethanol. DNA was extracted from each tentacle using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions. The cytochrome oxidase subunit I gene (COI) mitochondrial region was amplified using 1-2µl extracted DNA and MyTaq DNA polymerase mix (30µl reactions; Bioline UK (now Meridien Biosceince), with 10nmol each of the universal COI primers for invertebrates (LCO 1490 5'-GGTCAACAAATCATAAAGATATTGG-3'; HCO 2198 5'''''''-TAAACTTCAGGGTGACCAAAAAATCA-3') (Folmer et al. 1994). PCR conditions were: 94 °C for 5 minutes, 5 cycles of 94 °C for 1 min, 45 °C for 1.5 min, 72 °C for 1.5 min, followed by 30 cycles of 94 °C for 1 min, 50 °C for 1 min, 72 °C for 1 min and a final elongation stage of 5 min at 72 °C. The COI fragments were bi-directionally sequenced by Source Bioscience (Cambridge, UK). The species identity of each individual was analysed using Blast sequence similarity searching of INSDC (International Nucleotide Sequence Database Collaboration) ( https://www.insdc.org/).

Temperatures were raised at the same rate in all experiments (0.3 ± 0.1 °C d-1) until the incubation temperature was reached and subsequently monitored daily. Animals were held at temperatures of 6.0 and 8.0 °C, temperatures that were 0 to 4 °C above the long-term limits calculated from experiments with different rates of warming. Control animals were kept at 0.0 °C in the main holding aquarium.

Acute thermal limits: Individuals that survived beyond the duration of incubations at both 6.0 and 8.0 °C were then tested to see if acute thermal limits were elevated due to acclimation to these elevated incubation temperatures. These data were compared with acute thermal limits conducted on control individuals that had been kept in the aquarium at 0.0 °C. For the acute temperature ramping trials individuals were transferred to plastic jacketed tanks (Engineering Design and Plastics Ltd), whose jackets were filled with 25% v/v ethanol in water solution, that was heated or cooled by LTD20G thermocirculators (Grant Instruments). Temperatures were increased at 1.0 ± 0.1 °C d-1 until the last animal was no longer responding to the stimuli detailed above.

Acclimatisation in field animals was tested for in only one species, the common starfish O. validus. To test for field acclimatisation the assessment of CTmax of freshly collected O. validus was repeated between 2006 and 2015 in both summer and winter. There...(8)

Data quality:

All data have been quality checked by the data creators.

Metadata

File identifier

60b777b4-0bd6-48c3-a301-c700854fbfa1 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2024-03-11

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact