Date (Publication)

2024-05-08

Date (Creation)

2024-04-10

Citation identifier

https://catalogue.ceh.ac.uk/id/65c7085a-7bcf-481a-884a-8e1c9cfc395b

Citation identifier

doi: / 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b

Other citation details

Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of Edinburgh	Vale, P.	pedro.vale@ed.ac.uk	Author
University of Edinburgh	Vale, P.	pedro.vale@ed.ac.uk	Point of contact
University of Edinburgh	Kutzer, M.	mkutzer@exseed.ed.ac.uk	Author
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Custodian
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Publisher
University of Edinburgh		EMAIL NOT PROVIDED	Owner

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

Other restrictions

Other constraints

This resource is available under the terms of the Open Government Licence

Use constraints

Other restrictions

Other constraints

If you reuse this data, you should cite: Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b

Language

English

Character set

UTF8

Topic category

• Biota

Distribution format

Name	Version
Comma-separated values (CSV)

Distributor contact

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Distributor

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/data/65c7085a-7bcf-481a-884a-8e1c9cfc395b	Download the data

OnLine resource

Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/sd/65c7085a-7bcf-481a-884a-8e1c9cfc395b.zip	Supporting information

Hierarchy level

Non geographic dataset

Other

nonGeographicDataset

Statement

Sixty (60) lines from the DGRP were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed individually to infection overnight. Each treatment was replicated 5-10 times (707 samples in total). The experiment was split among 5 experimental blocks with a variable number of replicates tested in each, as described in the data file. Following infection (see above) pathogen shedding was measured by placing each individual fly inside a sterile Eppendorf and then quantifying the number of viable P. aeruginosa colony-forming units defecated each infected fly for 24h following oral infection. CFU (colony forming unit) plating was carried out on Pseudomonas sp. selective media (PIM) to select for P. aeruginosa CFUs only. Each fly was then transferred to Drosophila Activity Monitor (DAM) and the total number of activity events were measured for each fly individually measured at 1 min resolution until death (2500-~20,000 minutes). Data generated in the DAM included the total number of activity bouts recoded for each minute for the duration of the experiment (2500-~20,000 minutes). The values reported in the resource are the total number of activity bouts recoded per fly until death or until the end of the experiment. Mortality was recorded as the last detected movement in DAM.

Metadata

File identifier

65c7085a-7bcf-481a-884a-8e1c9cfc395b XML

Metadata language

English

Character set

8859 Part 1

Hierarchy level

Non geographic dataset

Hierarchy level name

nonGeographicDataset

Date stamp

2025-03-21T09:24:18

Metadata standard name

UK GEMINI

Metadata standard version

2.3

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Point of contact