BGS Hosted Metadata
Molecular analysis of Calanus from the Fram Strait 2019
Multidisciplinary field campaigns were carried out in the Fram Strait region during late summer 2019 (JR18007, 04/08/2019 to 28/08/2019) aboard RRS James Clark Ross. A motion-compensated Bongo net fitted with 200 um mesh was deployed at all locations to a maximum depth of 200 m or to within 10 m of the seabed.In the Arctic and sub-Arctic sectors adjacent to the Atlantic, three species of Calanus co-occur. However, their respective core distributions align with different water masses, with Calanus hyperboreus being a high-Arctic oceanic species, C. glacialis being associated with Arctic shelf waters, and C. finmarchicus dominating inflowing Atlantic Water.The proportions of these three species in each deployment was established through molecular analysis of the paired ethanol preserved sample. Subsamples of the ethanol sample were analysed using a 16S ribosomal RNA gene barcode (16SAR, 16SB2R primers) following an adapted protocol. Amplified DNA was sequenced using llumina high-throughput sequencing (HTS) platform. Resultant sequences processed through the Qiime pipeline, clustered into Operational Taxonomic Units at 97% homology and taxonomy was assigned using BLASTn (NCBI).
Funded by UKRI Natural Environment Research Council (NERC; NE/P006213/1, NE/P006353/1, NE/P006302/1, NE/ P005985/1 amongst others).
Simple
- Date (Creation)
- 2024-07-03
- Date (Revision)
- 2024-07-03
- Date (Publication)
- 2024-07-03
- Date (released)
- 2024-07-03
- Edition
-
1
- Unique resource identifier
- 65fb253d-2de3-4953-940a-525a1c3614d8
- Codespace
-
uuid
- Unique resource identifier
- GB/NERC/BAS/PDC/01858
- Codespace
- Unique resource identifier
- NE/P006213/1
- Codespace
-
award
- Unique resource identifier
- NE/P006353/1
- Codespace
-
award
- Unique resource identifier
- NE/P006302/1
- Codespace
-
award
- Other citation details
-
Please cite this item as: None
- Credit
-
No credit.
- Status
- Completed
- Point of contact
-
Organisation name Individual name Electronic mail address Role Plymouth Marine Laboratory
Parry, Helen
Author NERC EDS UK Polar Data Centre Point of contact
- Maintenance and update frequency
- As needed
- Maintenance note
- Completed
- Theme
-
-
16S ribosomal RNA
-
Calanus
-
bongo net
-
zooplankton
-
-
GEMET - INSPIRE themes, version 1.0
- Access constraints
- Other restrictions
- Other constraints
- no limitations to public access
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- License
- Other constraints
- This dataset is supplied under the Open Government Licence v3.0: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/
- Use constraints
- Other restrictions
- Other constraints
-
No restrictions apply.
- Unique resource identifier
- url
- Codespace
-
url
- Association Type
- Cross reference
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
-
- Biota
))
- Supplemental Information
-
It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.
- Title
-
European Petroleum Survey Group (EPSG) Geodetic Parameter Registry
- Date (Publication)
- 2008-11-12
- Cited responsible party
-
Organisation name Individual name Electronic mail address Role European Petroleum Survey Group
Publisher
- Unique resource identifier
- urn:ogc:def:crs:EPSG::3031
- Version
-
6.18.3
Distributor
- Distributor contact
-
Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Distributor
- Units of distribution
-
bytes
- Transfer size
- 1
- OnLine resource
-
Protocol Linkage Name WWW:LINK-1.0-http--link
https://www.ncbi.nlm.nih.gov/sra/PRJNA838801 Get Data
- Hierarchy level
- Dataset
- Statement
-
Methodology:
The 16S rRNA gene amplified using the primer pair 16SAR and 16S2R and sequenced using MiSeq by commercial contract (MR DNA, Texas, USA)
Multidisciplinary field campaigns were carried out in the Fram Strait region during late summer 2019 (JR18007, 04/08/2019 to 28/08/2019) aboard RRS James Clark Ross. A motion-compensated Bongo net fitted with 200 um mesh was deployed at all locations to a maximum depth of 200 m or to within 10 m of the seabed.In the Arctic and sub-Arctic sectors adjacent to the Atlantic, three species of Calanus co-occur. However, their respective core distributions align with different water masses, with Calanus hyperboreus being a high-Arctic oceanic species, C. glacialis being associated with Arctic shelf waters, and C. finmarchicus dominating inflowing Atlantic Water.The proportions of these three species in each deployment was established through molecular analysis of the paired ethanol preserved sample. Subsamples of the ethanol sample were analysed using a 16S ribosomal RNA gene barcode (16SAR, 16SB2R primers) following an adapted protocol. Amplified DNA was sequenced using llumina high-throughput sequencing (HTS) platform. Resultant sequences processed through the Qiime pipeline, clustered into Operational Taxonomic Units at 97% homology and taxonomy was assigned using BLASTn (NCBI).
Data collection:
motion-compensated Bongo net fitted with 200 um mesh
llumina high-throughput sequencing (HTS) platform
Metadata
- File identifier
- 65fb253d-2de3-4953-940a-525a1c3614d8 XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Hierarchy level name
-
dataset
- Date stamp
- 2024-07-03
- Metadata standard name
-
ISO 19115 Geographic Information - Metadata
- Metadata standard version
-
ISO 19115:2003(E)
- Metadata author
-
Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Point of contact
)))
