Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats

The dataset details surface sediment colloidal carbohydrate concentrations across six intertidal sites in the winter and summer of 2013. Colloidal carbohydrate concentrations in surface sediments (top 2mm) provide a quantitative measure of the extracellular polymeric substances (EPS) secreted by organisms that form the microphytobenthos (MPB) community. Three of the sites were in Morecambe Bay, North West England and three of the sites were in Essex, South East England, each of these sites consisted of a saltmarsh area and adjacent mudflat area, twenty two sampling quadrats were placed on each area. Five replicate sediment samples were taken at each quadrat and were analysed using the Dubois Phenol-Sulphuric Assay which involves spectrophotometer analysis of absorptions to determine colloidal carbohydrate concentrations. Values for colloidal carbohydrate concentrations are expressed as glucose equivalents in micrograms per gram of sediment. This data was collected as part of Coastal Biodiversity and Ecosystem Service Sustainability (CBESS): NE/J015644/1. The project was funded with support from the Biodiversity and Ecosystem Service Sustainability (BESS) programme. BESS is a six-year programme (2011-2017) funded by the UK Natural Environment Research Council (NERC) and the Biotechnology and Biological Sciences Research Council (BBSRC) as part of the UK's Living with Environmental Change (LWEC) programme. Full details about this dataset can be found at https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f

Date (Publication): 2016-04-25

Date (Creation): 2013-08-31

Citation identifier: https://catalogue.ceh.ac.uk/id/6c027bf9-b344-4178-b8b3-d78cf39f017f

Citation identifier: doi: / 10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f

Other citation details: Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre 10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of St Andrews	Maunder, J.	jm280@st-andrews.ac.uk	Point of contact
University of St Andrews	Maunder, J.	jm280@st-andrews.ac.uk	Author
University of St Andrews	Paterson, D.M.	dp1@st-andrews.ac.uk	Author
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Custodian
NERC Environmental Information Data Centre		info@eidc.ac.uk	Publisher
University of St Andrews		EMAIL NOT PROVIDED	Owner

Maintenance and update frequency: Not planned

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GEMET - Concepts, version 4.1.3

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Keywords

BESS
Coastal Biodiversity and Ecosystem Service Sustainability (CBESS)
sediment colloidal carbohydrates
surface sediment
mudflat
contact core
microphytobenthos
MPB
Dubois assay

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Other constraints: If you reuse this data, you should cite: Maunder, J., Paterson, D.M. (2016). Coastal Biodiversity and Ecosystem Service Sustainability (CBESS) surface sediment colloidal carbohydrate concentration in saltmarsh and mudflat habitats. NERC Environmental Information Data Centre https://doi.org/10.5285/6c027bf9-b344-4178-b8b3-d78cf39f017f

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Language: English

Character set: UTF8

Topic category

Oceans

Begin date: 2013-01-01

End date: 2013-08-31

Unique resource identifier: OSGB 1936 / British National Grid

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Comma-separated values (CSV)

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Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Distributor

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	https://data-package.ceh.ac.uk/data/6c027bf9-b344-4178-b8b3-d78cf39f017f	Download the data

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Protocol	Linkage	Name
	https://data-package.ceh.ac.uk/sd/6c027bf9-b344-4178-b8b3-d78cf39f017f.zip	Supporting information

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Title: Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication): 2010-12-08

Statement: Sediment colloidal carbohydrate concentration was determined through the collection of contact cores. Contact cores use liquid nitrogen to freeze the top 2mm of the sediment surface, excess unfrozen sediment is removed and the sample core is stored below -80 degree centigrade. The sample is weighed to determine wet weight and freeze dried to remove all water. Colloidal carbohydrate concentration is determined using the Dubois Phenol-Sulphuric assay. A sediment sub-sample (50-500mg, depending on site) is taken from dried contact core sediment and known volume of distilled water is added. Sample is vortexed and centrifuged then a set volume of supernatant is removed. Concentrated sulphuric acid and 5 percent phenol are added to the supernatant sample ensuring the ratio of sample: phenol: acid is kept at 1:1:5 by volume. Allow 35mins for colour to develop. Decant the samples into cuvettes and read the absorbance at 486.5nm on Thermo Biomate 5 spectrophotometer. Use figures from regression analysis of glucose standards to calculate colloidal carbohydrate concentration expressed as glucose equivalents in micrograms per gram (µg g-1). Note: zeroes indicate colloidal carbohydrate concentration below detectable level. Twenty two 1 x 1 m quadrats were randomly allocated to each mudflat and saltmarsh site using R (R Development Core Team, 2014) to specify four different spatial scales (A = 1 quadrat only, B = 3 quadrats at 1 m to 10 m apart, C = 6 quadrats at 10 m to 100 m apart, D = 12 quadrats at 100 m to 1000 m or site maximum).

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File identifier: 6c027bf9-b344-4178-b8b3-d78cf39f017f XML

Metadata language: English

Character set: 8859 Part 1

Hierarchy level: Dataset

Hierarchy level name: dataset

Date stamp: 2025-11-13T16:16:24

Metadata standard name: UK GEMINI

Metadata standard version: 2.3

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS Environmental Information Data Centre		info@eidc.ac.uk	Point of contact

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