Date (Creation)

2023-06-18

Date (Revision)

2023-06-18

Date (Publication)

2023-06-18

Date (released)

2023-06-18

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/7adc7b14-abae-4ab9-b60b-b9b6e0e9f320

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01749

Codespace

https://data.bas.ac.uk/

Unique resource identifier

NE/S007210/1

Codespace

award

Unique resource identifier

NE/N015894/1

Codespace

award

Unique resource identifier

NE/P006426/1

Codespace

award

Other citation details

Please cite this item as: Williams, T.J., Reed, A.J., Peck, L.S., Godbold, J.A., & Solan, M. (2023). Measurements for a 92 day climate manipulation experiment on replicate macrofaunal mesocosms collected on the cruise JR18006 in the Western Barents Sea and at Rothera research station in 2019 (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/7adc7b14-abae-4ab9-b60b-b9b6e0e9f320

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
University of Southampton	Williams, Thomas J.		Author
University of Southampton	Reed, Adam J.		Author
British Antarctic Survey	Peck, Lloyd S.		Author
University of Southampton	Godbold, Jasmin A.		Author
University of Southampton	Solan, Martin		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
• EARTH SCIENCE > Oceans > Ocean Chemistry > Carbon Dioxide
• EARTH SCIENCE > Oceans > Ocean Temperature

Theme

• Antarctic

• Arctic

• bioirrigation

• bioturbation

• climate change

• ecosystem function

• experiment

• mesocosm experiment

Place

• Hamble, UK

• Barents Sea, Antarctic Barents Sea

• Rothera Point, Antarctic Peninsula Antarctica

GEMET - INSPIRE themes, version 1.0

• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC data policy and supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

No restrictions apply.

Unique resource identifier

url

Codespace

url

Association Type

dependency

Unique resource identifier

doi

Codespace

doi

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2019-09-21

End date

2020-01-21

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Individuals of Astarte crenata, Ctenodiscus crispatus and Cistenides hyperborea were obtained from replicate SMBA (Scottish Marine Biological Association) box cores and 15 minute Agassiz trawls in the Barents Sea (stations B13, 74.3 degrees N, 30.0 degrees E; B16, 80.3 degrees N, 30.0 degrees E; JCR18006, RSS James Clark Ross) in July 2019. Individuals of the Aequiyoldia eightsi and Laternula elliptica were collected by SCUBA-assisted divers at Rothera Point, Adelaide Island, West Antarctic Peninsula (67.3 degrees S, 68.1 degrees W) in March-April 2019. Surficial sediment (less than 5 cm depth) for the Arctic species was collected using SMBA box cores at stations B13, B14 (76.3 degrees N, 30.3 degrees E) and B16 in July 2019 and, for the Antarctic species, by hand from the Hamble, UK (50.9 degrees N, 1.3 degrees W). Sediment was sieved (500 micrometer mesh) within a seawater bath to retain the fine fraction and remove macrofauna and debris.

Species were distributed across 42 clear acrylic aquaria (internal LWH: 12 x 12 x 33 cm, 3 replicates treatment-1: species × location × climate scenario), designed to accommodate the appropriate density (Arctic, 2 ind. mesocosm-1; Antarctic, 1 ind. Mesocosm-1; size and burrowing requirements of each species (sediment depth: A. crenata, C. crispatus & C. hyperborea, 16 cm; A. eightsi, 12cm; L. elliptica, 19cm), and exposed to ambient (1 ± 0.5°C, around 400 ppm [CO2]) and indicative near-future (3 +/- 0.5 degrees Celsius, around 550 ppm [CO2]) environmental conditions. Aquaria were randomly placed within one of two insulated seawater reservoirs (LWH: 1.2 x 1.2 x 0.8m, Tanks Direct, UK). All aquaria were overlain with seawater (salinity 33, 10 micrometer sand filtered, UV sterilized) to a combined sediment and water depth of 31 cm and maintained in the dark. After acclimation to ambient mesocosm conditions (21 days, 09-29/09/2019), the water temperature and atmospheric CO2 of the aquaria in the future treatment was gradually increased at 0.5 degrees Celsius and 50 ppm increments every 7 days to reach the desired near-future climate conditions. During both the acclimation and experimental period (92 days, 21/10/2019 - 21/01/2020), C. crispatus and C. hyperborea were fed ad libitum once a week with commercially available fish food (Aquarian Tropical Flake; 0.03 g), and A. crenata, A. eightsi and L. elliptica were fed ad libitum three times per week with 100 ml of precultured phytoplankton (33:33:33 mix, Isochrysis sp., Tetraselmis sp., and Phaeodactylum sp.; Algal Culture Laboratory, National Oceanography Centre Southampton). To avoid accumulation of excess food and associated nutrients, partial seawater changes (weekly 50 percentage seawater exchange) were undertaken.

Temperature, pH, salinity and total alkalinity were periodically measured across all experimental mesocosms (T,pH,S: weekly, AT: week 2, week 6, week 11). Total alkalinity analysis followed the standard HCl titration protocol of the Carbonate Facility at the University of Southampton. From which, DIC, [pCO2], [calcite], [aragonite], [HCO3] and [CO3] were calculated using the CO2calc software.

CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption were measured continuously in the ambient air and future climate regime gas mixture from the start (21/10/2019) to end (21/01/2020) of the experimental period. All measurements were calculated using an Infra-red gas analyzer LiCOR LI-820 model and recorded using the interface software on a standard laptop.

Behavioural activity was quantified using measurements of movement and burial behaviour at the sediment surface. Individuals (morphology, +/- 0.01 mm; blotted wet weight, +/- 0.001 g) were placed in temperature-acclimatised viewing trays containing sediment (depth 5 cm) overlain with sea water (depth 3 cm) and viewed (less ...(31)

Data collection:

Behaviour: Logitech C920 HD Pro Webcam, 1080p

Behaviour: SkyStudioPro time-lapse software V 1.1.0.30

Behaviour: VLC Media Player V 3.0.12

Sediment particle reworking: Canon 400D digital SLR camera

Sediment particle reworking: ImageJ (version 1.47s)

Bioirrigation: Tecator flow injection auto-analyser (FIA Star 5010 series)

Nutrients: Lachat Quikchem 8500 flow-injection auto-analyser

Total Alkalinity: Apollo SciTech Alkalinity Titrator AS-ALK2

Salinity: WTW (TM) TetraCom (TM) 325 Standard temperature-conductivity combination electrode

Temperature: NBS scale, Mettler-Toledo InLab Expert Pro temperature-pH combination electrode

pH: NBS scale, Mettler-Toledo InLab Expert Pro temperature-pH combination electrode

pCO2, CO3, HCO3, Ωcalcite, Ωaragonite, TCO2: Calculated using the CO2calc software [version 4.0.9]

CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption : Infra-red gas analyzer LiCOR Li-820

CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption: ACER laptop CO2(ppm), H2O(ppt), H2O(C), Cell_Temperature(C), Cell_Pressure(kPa), CO2_Absorption and H2O_Absorption: LiCOR Windows software

Sediment: Malvern Mastersizer 2000 He-Ne LASER diffraction sizer

Data quality:

Standard protocols were followed and data entry double checked by independent person. Missing values for behavioural activity (response rate, burial rate) relate to the individual not completing the associated activity within the 60 minute timeframe. Missing biomass data is due to mortalities. Original unedited raw videos and processed, stitched together timelapses are provided. Original unedited raw fSPI images and processed, stitched together fSPI profiles are provided.

3 replicate water samples were taken for each independent treatment level in the experiments. Standards and blanks were utilised when analysing total alkalinity as per the HCl protocol. Standard protocols were followed and data entry double checked by independent person.

LiCOR instruments were calibrated 3 months prior to the start of the experimental period.

Metadata

File identifier

7adc7b14-abae-4ab9-b60b-b9b6e0e9f320 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2023-06-18

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact