Date (Creation)

2022-08-05

Date (Revision)

2022-08-05

Date (Publication)

2022-08-05

Date (released)

2022-08-05

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/87fd0d61-7405-4a66-9620-2be10c25d452

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01655

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Newsham, K. (2022). Microscopy data on mycothalli in 16 leafy liverwort species from the Thatcher Peninsula, South Georgia (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/87fd0d61-7405-4a66-9620-2be10c25d452

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Newsham, Kevin K		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Ecological Dynamics > Symbiosis
• EARTH SCIENCE > Biosphere > Fungi

Theme

• Jungermanniidae

• South Georgia

• leafy liverwort

• mycothalli

• symbiosis

Place

• Thatcher Peninsula South Georgia Island

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/

Use constraints

Other restrictions

Other constraints

This data is governed by the NERC data policy and supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

No restrictions apply

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2011-11-01

End date

2016-02-29

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

In October to November 2011 and January to February 2016, 83 specimens of 16 leafy liverwort species in the Adelanthaceae, Anastrophyllaceae, Cephaloziaceae, Cephaloziellaceae, Lophoziaceae, Lophocoleaceae, Plagiochilaceae and Schistochilaceae were collected from 12 sites on the Thatcher Peninsula.

The specimens, which were air dried at room temperature in clean paper packets within several hours of collection, have been deposited in the British Antarctic Survey herbarium (AAS see https://data.bas. ac.uk/metadata.php?id=GB/NERC/BAS/AEDC/00023). Dried plants were rehydrated in water and cleared in 10% KOH for 24 h at room temperature. All plants were then rinsed thoroughly with water and were bleached in a mixture of 30% hydrogen peroxide and 3% ammonium hydroxide (10:1 v/v) for up to 10 min. The plants were then washed thoroughly with water, were acidified in 5% lactic acid for 1 h and were stained with 0.01% aniline blue in lactic acid for 24 h. They were then removed from the staining solution, excess stain was drawn off on absorbent paper, and the plants were destained for at least 24 h in 80% lactic acid. From each sample, between 10 to 20 stems of each species, and at least 40 stems of Cephalozia badia, Cephaloziella varians and Clasmatocolea humilis, were then lightly squashed in 80% lactic acid on glass slides prior to observation under UV epifluorescence at x400 magnification (BX51 microscope, Olympus UK Ltd). The line intersection method described by McGonigle et al. (1990) was used to calculate the percentages of stem length colonised (SLC) by stained hyphal coils in rhizoid bases and stem epidermal cells, stained septate hyphae in stem epidermal cells, dark septate hyphae on stem surfaces, and dark septate coils in rhizoid bases and stem epidermal cells. A total of 4,442 intersections were examined, with up to 119 intersections being scored in each sample. The percentage of at least 30 rhizoids in each specimen that were colonised by hyphae was also recorded, with a total of 2,541 rhizoids being examined. Insufficient rhizoids were observed on the stems of Chiloscyphus koeppensis or Pachyglossa spegazziniana to derive means for rhizoid colonisation.

Instrumentation: BX51 compound microscope fitted with a UPlanApo x40 objective lens, a 100-W mercury short arc lamp and an ultraviolet fluorescence filter cube (U-MWU2, consisting of a BP 330-385 excitation filter, a DM 400 dichromatic mirror and an LP 420 emission filter; Olympus Life Science, Tokyo, Japan).

Data collection:

BX51 compound microscope fitted with a UPlanApo x40 objective lens, a 100-W mercury short arc lamp and an ultraviolet fluorescence filter cube (U-MWU2, consisting of a BP 330-385 excitation filter, a DM 400 dichromatic mirror and an LP 420 emission filter; Olympus Life Science, Tokyo, Japan).

Data quality:

Raw data are shown. The data have not been cleaned. Missing data in the Rhizoids colonised (%) column (indicating that no rhizoids were observed on the stems of plants) are indicated by NA.

Metadata

File identifier

87fd0d61-7405-4a66-9620-2be10c25d452 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2022-08-05

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact