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Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford

The data comprises both compositional data and growth assay data from an experiment of wild bacterial communities in Wytham Woods, Oxford. The communities live in puddles of water in tree-holes formed by the roots of beech trees. We manipulated the pH of tree-holes during the experiment for comparison with control tree-holes that were unmanipulated. Compositional data comprises the frequency of different Amplicon Sequencing Variants and derived measures of species diversity for samples taken over time (up to 12 weeks from the start of the experiment). The growth assay data comprises measurements of growth rates of community samples and isolates back in the laboratory, when grown on beech tea media manipulated to have different pHs. Full details about this dataset can be found at https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30

Simple

Date (Publication)
2025-10-20
Citation identifier
https://catalogue.ceh.ac.uk/id/9d64f2bd-2918-416f-b08c-3369edbc1b30
Citation identifier
doi: / 10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30
Other citation details

Barraclough, T.G., Sant, D.G., Wong, E.L.Y. (2025). Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford. NERC EDS Environmental Information Data Centre 10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30

Point of contact
Organisation name Individual name Electronic mail address Role
University of Oxford

Timothy Giles Barraclough

tim.barraclough@biology.ox.ac.uk

Point of contact
University of Oxford Barraclough, T.G.

tim.barraclough@biology.ox.ac.uk

Author
Rutgers, The State University of New Jersey Sant, D.G.

duhita.sant@rutgers.edu

Author
Senckenberg Biodiversity and Climate Research Centre

Wong, E.L.Y.

edgar.wong@senckenberg.de

Author
University of Oxford

EMAIL NOT PROVIDED

Owner
NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Publisher
NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Custodian

GEMET - INSPIRE themes, version 1.0

  • Environmental Monitoring Facilities

GEMET - Concepts, version 4.1.3

  • bacterium
  • evolution
  • ecological community
  • field study
  • field experiment
  • microbiology
  • deciduous tree
Keywords
  • Biodiversity
  • Evolutionary ecology
  • pH
Access constraints
Other restrictions
Other constraints
no limitations
Use constraints
Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
Other restrictions
Other constraints

If you reuse this data, you should cite: Barraclough, T.G., Sant, D.G., Wong, E.L.Y. (2025). Compositional and growth assay data for tree-hole bacteria communities under differing pH conditions, sampled summer 2021, Wytham Woods, Oxford. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/9d64f2bd-2918-416f-b08c-3369edbc1b30

Spatial representation type
Text, table
Language
English
Character set
UTF8
Topic category
  • Biota
Begin date
2021-05-11
End date
2021-09-29
N
S
E
W
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Unique resource identifier
OSGB 1936 / British National Grid
Distribution format
Name Version

Comma-separated values (CSV)

Distributor contact
Organisation name Individual name Electronic mail address Role

NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Distributor
OnLine resource
Protocol Linkage Name
https://data-package.ceh.ac.uk/sd/9d64f2bd-2918-416f-b08c-3369edbc1b30.zip

Supporting information

OnLine resource
Protocol Linkage Name
https://data-package.ceh.ac.uk/data/9d64f2bd-2918-416f-b08c-3369edbc1b30

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Hierarchy level
Dataset
Other

dataset

Conformance result

Title

Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services

Date (Publication)
2010-12-08
Statement

A field experiment was conducted in summer 2021 that surveyed bacterial communities in tree-hole puddles formed by beech tree roots at Wytham Woods, Oxford. The experiment added lime (alkali) to a set of tree-holes and compared changes in bacterial communities to control tree-holes without liming. Samples were collected before and after the manipulation, and some of these were used for growth assays in the laboratory: growing different combinations of isolates and communities on beech tea medium with modified pH.

Metadata

File identifier
9d64f2bd-2918-416f-b08c-3369edbc1b30 XML
Metadata language
English
Character set
8859 Part 1
Hierarchy level
Dataset
Hierarchy level name

dataset

Date stamp
2025-11-13T16:14:51
Metadata standard name
UK GEMINI
Metadata standard version

2.3

Metadata author
Organisation name Individual name Electronic mail address Role
NERC EDS Environmental Information Data Centre

info@eidc.ac.uk

Point of contact
 
 

Overviews

Spatial extent

thumbnail

Keywords

GEMET - INSPIRE themes, version 1.0

Environmental Monitoring Facilities


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