Date (Creation)

2024-07-22

Date (Revision)

2024-07-22

Date (Publication)

2024-07-22

Date (released)

2024-07-22

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/b354cde1-8cc6-4b29-a1b2-e21fa3e412e9

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/01866

Codespace

https://data.bas.ac.uk/

Other citation details

Please cite this item as: Frontier, N., Clark, M.S., & Peck, L.S. (2024). Development and molecular barcoding of Terebellidae polychaetes from Rothera Research Station, Antarctic Peninsula, Antarctica (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/b354cde1-8cc6-4b29-a1b2-e21fa3e412e9

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Frontier, Nadia		Author
British Antarctic Survey	Clark, Melody S		Author
British Antarctic Survey	Peck, Lloyd S		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Biosphere > Zoology > Invertebrates
• EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
• EARTH SCIENCE > Oceans > Marine Biology

Theme

• Terebellidae

• larval development

• metatrocophore

• nectochaete

• trocophore

Place

• Ryder Bay, Antarctic Peninsula Antarctica

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes
• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

no limitations to public access

Access constraints

Other restrictions

Other constraints

no limitations

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Data supplied under Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

No restrictions apply.

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota

Begin date

2021-04-22

End date

2021-04-22

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

Egg samples were collected on 2021-04-22 from Troval dive site, Ryder Bay, using SCUBA. Specimens were maintained in ambient water temperature during transit from the dive site to the Aquarium. Egg samples were transferred into a through-flow aquarium tank where they remained for seven months.

Specimens were checked each week and photographed upon noticeable changes in their development. In the Bonner Laboratory aquarium, a subsample of the egg mass was pipetted from the mucus mass and transferred to a petri dish. A portable dissection microscope (Meiji EMZ-TR) was temporarily installed in the aquarium.

Photographs were collected using a simple system of aligning an Olympus TG4 camera in the lens viewer, in the absence of a suitable aquarium microscope with a fitted camera.

Samples were quickly returned to the aquarium tank.

Molecular barcoding using the 18SrRNA gene was performed to identify the larvae. Briefly DNA was extracted from the larvae using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions. Amplification of a 560bp fragment of the 18S rRNA was performed using the primers NSF4 (CTGGTTGATYCTGCCAGT) and NSR581 (ATTACCGCGGCTGCTGGC) and 2x MyTaq HS Readymix (Meridian Biosciences) according to manufacturer's instructions. PCR cycling conditions were as follows: 95 degrees C for 5 mins, the 35 cycles of 95 degrees C for 20 secs; 60 degrees C for 20 secs; 72 degrees C for 30 secs and a final extension of 72 degrees C for 10 mins. The PCR product was Sanger sequenced by Source Bioscience (UK). Blast sequence similarity searching revealed that it was highly likely that these larvae were an uncharacterised Terebellidae species. In the absence of any associated adults for detailed taxonomy, more detailed identification to species level was not possible. The 18SrRNA sequence is available from NCBI under Accession number PP908343.

Metadata

File identifier

b354cde1-8cc6-4b29-a1b2-e21fa3e412e9 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2024-07-22

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact