BGS Hosted Metadata
Survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions
This dataset contains information relating to the survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions. The data encompass bacterial strain identity, material type, timepoints (days), bacterial concentration (CFU/ml) and survival percentages. Data were sampled over a 28-day period using culture-based approaches; and the ability of isolates to cause disease following their recovery was determined through the use of a Galleria mellonella model. The ability of isolates to persist on materials, dissociate from materials into water bodies, and recolonise materials was also determined. This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1]. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b
Simple
- Date (Publication)
- 2025-06-12
- Citation identifier
- https://catalogue.ceh.ac.uk/id/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b
- Citation identifier
- doi: / 10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b
- Other citation details
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Ormsby, M., Woodford, L., White, H., Fellows, R., Oliver, D., Quilliam, R. (2025). Survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions. NERC EDS Environmental Information Data Centre 10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Stirling Michael Ormsby
Point of contact University of Stirling Ormsby, M. Author University of Stirling Woodford, L. Author University of Stirling White, H.
Author University of Stirling Fellows, R. Author University of Stirling Oliver, D. Author University of Stirling Quilliam, R. Author NERC EDS Environmental Information Data Centre Custodian NERC EDS Environmental Information Data Centre Publisher University of Stirling EMAIL NOT PROVIDED
Owner
- Keywords
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- Environmental risk
- Climate and climate change
- Water quality
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Biofilm
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Human Pathogens
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Plastic Pollution
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Plastisphere
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Public Health
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Ormsby, M., Woodford, L., White, H., Fellows, R., Oliver, D., Quilliam, R. (2025). Survival and persistence of clinically relevant strains of toxigenic Vibrio cholerae on plastic and vegetation, under environmentally relevant conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b
- Language
- English
- Character set
- UTF8
- Topic category
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- Environment
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Data were generated through laboratory experiments investigating the persistence, virulence, and survival of clinically relevant strains of toxigenic Vibrio cholerae. Bacteria were cultured on materials (Polyethylene [PE], polypropylene [PP] and banana leaf [BL]) and analysed for persistence over time (up to 28 days) and virulence using Galleria mellonella larvae. Materials (PE, PP and BL) were prepared and inoculated under controlled laboratory conditions. V. cholerae strains were inoculated onto materials in nutrient-rich and surface water environments. Sampling was taken at defined time points (7, 14, 21, 28 days). G. mellonella larvae were used to evaluate virulence, and survival was assessed over 72 h. The experiments utilised standard laboratory equipment such as 96-well plates and incubators. G. mellonella larvae were sourced from a local supplier and used within one week of delivery. Instrumentation used for bacterial enumeration, including the plate reader, was calibrated according to standard laboratory protocols. The data underwent rigorous quality control through replication (biological triplicates), statistical analysis (ANOVA), and controlled experimental conditions (e.g., temperature and material consistency). Statistical methods including ANOVA and Tukey’s post-hoc tests were applied to assess treatment effects and survival outcomes.
Metadata
- File identifier
- b9bfdb6f-5e5e-496f-bf0c-bb9931f6046b XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2025-11-13T16:22:50
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
