BGS Hosted Metadata
Krill genetic profiling from samples collected in the Southern Ocean and South Atlantic during the Discovery 2010 cruise JR177
Genetic profiling data relating to studies on Antarctic krill, Euphausia superba, that document the sequence of expression of genes over the moult cycle and the spatial-temporal expression of clock genes. This work was carried out to examine rhythmic behaviour patterns in this species - namely diel vertical migration and the moult cycle - and the functioning of the genes that underlie these behaviours.
Circadian entrainment experiments were carried out twice during the Discovery 2010 summer cruise (cruise no JR177) using krill caught in nets at latitudes of 60S and 52S. Krill samples from each net were processed and preserved for subequent analysis using molecular biology technique to isolate canonical clock genes.
Simple
- Date (Creation)
- 2012-03-07
- Date (Revision)
- 2012-03-07
- Date (Publication)
- 2012-03-07
- Date (released)
- 2012-03-07
- Edition
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1
- Unique resource identifier
- c38663cd-2fdf-436a-af4b-c28da67707ba
- Codespace
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uuid
- Unique resource identifier
- GB/NERC/BAS/PDC/00646
- Codespace
- Other citation details
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Please cite this item as: None
- Credit
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No credit.
- Status
- Completed
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Leicester
Rosato, Ezio
Author British Antarctic Survey Tarling, Geraint
Author Shreeve, Rachael
Author University of Leicester
Kyriacou, Charalambos
Author University of Leicester
Gaten, Edward
Author NERC EDS UK Polar Data Centre Point of contact
- Maintenance and update frequency
- As needed
- Maintenance note
- Completed
- Global Change Master Directory (GCMD) Science Keywords
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- EARTH SCIENCE > Agriculture > Animal Science > Animal Ecology and Behavior
- EARTH SCIENCE > Biosphere > Animal Taxonomy > Crustaceans
- EARTH SCIENCE > Oceans > Aquatic Sciences > Fisheries
- EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
- EARTH SCIENCE > Oceans > Aquatic Sciences
- EARTH SCIENCE > Oceans > Marine Biology
- Theme
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Sea regions
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Euphausia
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animal behaviour
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circadian
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clock-genes
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krill
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microarray
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moult cycle
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phylogenetics
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- Place
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Near South Orkney Islands Southern Ocean
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North-West of South Georgia South Atlantic Ocean
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GEMET - INSPIRE themes, version 1.0
- Access constraints
- Other restrictions
- Other constraints
- no limitations to public access
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- License
- Other constraints
- This dataset is supplied under the Open Government Licence v3.0: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/
- Unique resource identifier
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url
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- Cross reference
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- url
- Codespace
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url
- Association Type
- Cross reference
- Unique resource identifier
- url
- Codespace
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url
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- Cross reference
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url
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url
- Association Type
- Cross reference
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- url
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url
- Association Type
- Cross reference
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Oceans
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- Begin date
- 2007-12-31
- End date
- 2011-06-30
- Supplemental Information
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It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.
- Title
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European Petroleum Survey Group (EPSG) Geodetic Parameter Registry
- Date (Publication)
- 2008-11-12
- Cited responsible party
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Organisation name Individual name Electronic mail address Role European Petroleum Survey Group
Publisher
- Unique resource identifier
- urn:ogc:def:crs:EPSG::3031
- Version
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6.18.3
Distributor
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Distributor
- Units of distribution
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bytes
- Transfer size
- 1
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--link
http://www.ncbi.nlm.nih.gov/dbEST/ Get Data
- Units of distribution
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bytes
- Transfer size
- 1
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--link
http://www.ncbi.nlm.nih.gov/genbank/ Get Data
- Units of distribution
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bytes
- Transfer size
- 1
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--link
http://www.ebi.ac.uk/arrayexpress/ Get Data
- Hierarchy level
- Dataset
- Statement
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Methodology:
An entrainment tank containing around 250 l of flowing seawater was used for studying the spatial and temporal expression of clock genes. The animals were maintained under a controlled light/environmental regime that mimicked that normally experienced by swarms at the latitude from which they were taken. After 2 days entrainment, a sample of krill (usually 10 animals) was taken at 3-hourly intervals over a period of 48 hours; these were either preserved in RNAlater (a commercially available solution for long term storage of tissues without deterioration of their RNA) or were frozen directly at -80C. During the next 24 hours samples were taken at 6-hourly intervals and fixed in PFA as above. The lights were switched off and on the 2nd, 3rd and 4th day in total darkness, the above sampling regime was repeated.
The circadian entrainment experiment was carried out twice, using animals from nets at latitudes of 60S and 52S. A total of 1,090 preserved specimens were returned to the UK for subsequent molecular genetic analysis.
A second entrainment tank was used to study the moult cycle genes. Up to 20 animals were removed each day at the same time, measured, moult staged and the heads preserved in RNAlater for subsequent use in the microarray studies to isolate the genes involved in the moult cycle. A total of 590 krill in RNAlater were returned to the UK at -20C for further analysis. The ambient water temperatures for these latitudes were taken from CTD data.
Data collection:
See the following for details:
Seear PJ, Tarling GA, Burns G, Goodall-Copestake WP, Gaten E, Ozkaya O, Rosato E (2010). Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba) BMC Genomics, 11: 582.
Seear P J, Tarling GA, Teschke M, Meyer B, Thorne MAS , Clark MS, Gaten E, Rosato E (2009) Effects of simulated light regimes on gene expression in Antarctic krill (Euphausia superba Dana) Journal of Experimental Marine Biology and Ecology, 381: 57-64
Metadata
- File identifier
- c38663cd-2fdf-436a-af4b-c28da67707ba XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2012-03-07
- Metadata standard name
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ISO 19115 Geographic Information - Metadata
- Metadata standard version
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ISO 19115:2003(E)
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Point of contact
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