BGS Hosted Metadata
Bacterial diversity on Gourlay Snowfield and Tuva Glacier, Signy Island, Antarctica, 2012-2013
Between December 2012 and March 2013, snow measurements were conducted in 3 snow pits at both Gourlay Snowfield and Tuva Glacier, Signy Island, to determine the bacterial diversity within the snowpacks. Sites are denoted 'TX' and 'GY', where 'X' and 'Y' are numbers representing one of nine snowpits in a grid at Tuva and Gourlay respectively. Snow samples of the 'top' layer were taken from the surface snow layer at a depth of 0 to 20 cm from the surface; snow samples of the middle 'mid' layer were taken from 20 cm to the bottom of the snow pit; and samples from the 'ice' layer were taken from the superimposed ice at the bottom of the snow pit. Snow and ice samples were collected from these pits and transported frozen to the UK for further analysis.
Funding was provided by the NERC grants NE/H014446/1 and NE/H014802/1.
Simple
- Date (Creation)
- 2017-05-08
- Date (Revision)
- 2017-05-08
- Date (Publication)
- 2017-05-08
- Date (released)
- 2017-05-08
- Edition
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1
- Unique resource identifier
- https://doi.org/10.5285/da355c78-3039-4a1d-b257-664e0f4dfd24
- Codespace
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doi
- Unique resource identifier
- GB/NERC/BAS/PDC/00933
- Codespace
- Unique resource identifier
- NE/H014446/1
- Codespace
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award
- Unique resource identifier
- NE/H014802/1
- Codespace
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award
- Other citation details
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Please cite this item as: None
- Credit
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No credit.
- Status
- Completed
- Point of contact
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Organisation name Individual name Electronic mail address Role British Antarctic Survey Convey, Pete
Author University of Bristol
Sabacka, Marie
Author University of Sheffield
Hodson, Andy
Author British Antarctic Survey Pearce, David
Author NERC EDS UK Polar Data Centre Point of contact
- Maintenance and update frequency
- As needed
- Maintenance note
- Completed
- Theme
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Bacterial production
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Glacier
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Primary production
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Sequencing
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Signy Island
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Snow
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Superimposed ice
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- Place
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Gourlay Snowfield and Tuva Glacier, Signy Island Antarctica
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GEMET - INSPIRE themes, version 1.0
- Access constraints
- Other restrictions
- Other constraints
- no limitations to public access
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- License
- Other constraints
- Open Government Licence v3.0
- Use constraints
- Other restrictions
- Other constraints
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This data is governed by the NERC Data Policy: https://www.ukri.org/who-we-are/nerc/our-policies-and-standards/nerc-data-policy/
- Use constraints
- Other restrictions
- Other constraints
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This data is governed by the NERC data policy and supplied under Open Government Licence v.3
- Use constraints
- Other restrictions
- Other constraints
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Requests for data can be made via the UK Polar Data Centre (UK PDC) at BAS.
- Unique resource identifier
- url
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url
- Association Type
- Cross reference
- Unique resource identifier
- url
- Codespace
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url
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- Cross reference
- Unique resource identifier
- url
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url
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- Cross reference
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- url
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url
- Association Type
- Cross reference
- Unique resource identifier
- url
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url
- Association Type
- Larger work citation
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Biota
- Climatology, meteorology, atmosphere
- Environment
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- Begin date
- 2012-12-01
- End date
- 2013-03-31
- Supplemental Information
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It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.
- Title
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European Petroleum Survey Group (EPSG) Geodetic Parameter Registry
- Date (Publication)
- 2008-11-12
- Cited responsible party
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Organisation name Individual name Electronic mail address Role European Petroleum Survey Group
Publisher
- Unique resource identifier
- urn:ogc:def:crs:EPSG::3031
- Version
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6.18.3
Distributor
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Distributor
- Units of distribution
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bytes
- Transfer size
- 1
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--link
https://ramadda.data.bas.ac.uk/repository/entry/show/?entryid=da355c78-3039-4a1d-b257-664e0f4dfd24 Get Data
- Hierarchy level
- Dataset
- Statement
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Methodology:
Melted snow and ice (500-1500 mL) was filtered onto 0.2µm Whatman® Nucleopore using a sterilized filtration unit. DNA was isolated from the filter using MoBio PowerWater® DNA isolation kit and following the instruction manuals.
The highly variable V3/V4 region of the 16S rDNA was amplified with bacterial primers S-D-Bact-0341-b-S-17 forward and S-D-Bact-0785-a-A-21 reverse, with overhang illumina adaptor attached to the primer sequences, creating a single amplicon of ~460 bp (Klindworth et al., 2013). The reaction was carried out in 50 µl volumes containing 0.3 mg/ml Bovine Serum Albumin, 250 uM dNTPs, 0.5 uM of each primer, 0.02 U Phusion High-Fidelity DNA Polymerase (Finnzymes OY, Espoo, Finland) and 5x Phusion HF Buffer containing 1.5mM MgCl2. The following PCR conditions were used: initial denaturation at 95C for 5min, followed by 25 cycles consisting of denaturation (95oC for 40 s), annealing (55oC for 2 min) and extension (72oC for 1 min) and a final extension step at 72oC for 7 min. Samples were sequenced using illumina MiSeq platform at Liverpool Centre for Genomics Research and generated 2 x 300 bp overlapping pair-end reads.
The 16S sequences were further processed using mothur (v. 1.35) pipeline (Schloss et al., 2009). Chimeric sequences were identified and removed using UCHIME (Edgar et al., 2011). Reads were clustered into operational taxonomical units (OTUs), based on at least 97% sequence similarity, and assigned taxonomical identification against SILVA bacterial database (Quast et al., 2013).
Data collection:
Instruments used include:
PCR machine
MiSeq illumina sequencer
Metadata
- File identifier
- da355c78-3039-4a1d-b257-664e0f4dfd24 XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2017-05-08
- Metadata standard name
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ISO 19115 Geographic Information - Metadata
- Metadata standard version
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ISO 19115:2003(E)
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS UK Polar Data Centre Point of contact
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