Date (Creation)

2025-10-08

Date (Revision)

2025-10-08

Date (Publication)

2025-10-08

Date (released)

2025-10-08

Edition

1.0

Unique resource identifier

https://doi.org/10.5285/e115d087-e3e4-4794-b4e0-18262b8e6400

Codespace

doi

Unique resource identifier

GB/NERC/BAS/PDC/02090

Codespace

https://data.bas.ac.uk/

Unique resource identifier

NE/W004933/1

Codespace

award

Other citation details

Please cite this item as: Linse, K., Ford, D., Blackbird, S.J., Brix, S., Freer, J.J., Jeffreys, R., Schmidt, L.A., & Mayor, D.J. (2025). Benthic, epibenthic and pelagic marine invertebrate specimens collected during PS136 in the Arctic Fram Strait in 2023 and their individual lipid profiles and bulk isotopes (Version 1.0) [Data set]. NERC EDS UK Polar Data Centre. https://doi.org/10.5285/e115d087-e3e4-4794-b4e0-18262b8e6400

Credit

No credit.

Status

Completed

Point of contact

Organisation name	Individual name	Electronic mail address	Role
British Antarctic Survey	Linse, Katrin		Author
University of Exeter	Ford, Dewi		Author
University of Liverpool	Blackbird, Sabena J.		Author
Senckenberg am Meer	Brix, Saskia		Author
British Antarctic Survey	Freer, Jennifer J.		Author
University of Liverpool	Jeffreys, Rachel		Author
University of Rostock	Schmidt, Lydia A.		Author
University of Exeter	Mayor, Daniel J.		Author
NERC EDS UK Polar Data Centre		PDCServiceDesk@bas.ac.uk	Point of contact

Maintenance and update frequency

As needed

Maintenance note

Completed

Global Change Master Directory (GCMD) Science Keywords

• EARTH SCIENCE > Oceans > Marine Biology > Marine Invertebrates
• EARTH SCIENCE > Oceans > Ocean Chemistry > Hydrocarbons
• EARTH SCIENCE > Oceans > Ocean Chemistry > Stable Isotopes
• EARTH SCIENCE > Oceans > Marine Environment Monitoring

Theme

• δ15N signatures

• Calanus

• Macrobenthos

• benthopelagic coupling

• fatty acid and alcohol biomarkers

Place

• Greenland Sea - Fram Strait - Molloy Deep Arctic

GEMET - INSPIRE themes, version 1.0

• Oceanographic geographical features

Access constraints

Other restrictions

Other constraints

public access limited according to Article 13(1)(e) of the INSPIRE Directive

Use constraints

License

Other constraints

Open Government Licence v3.0

Use constraints

Other restrictions

Other constraints

Dara are supplied under Open Government Licence v.3

Use constraints

Other restrictions

Other constraints

Data are embargoed until publication of Dewi et al. (submitted June 2025) or made freely available after 2 years (2027).

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

url

Codespace

url

Association Type

Cross reference

Unique resource identifier

doi

Codespace

doi

Association Type

Cross reference

Spatial representation type

Text, table

Language

English

Character set

UTF8

Topic category

• Biota
• Environment
• Oceans

Begin date

2023-05-29

End date

2023-06-11

Supplemental Information

It is recommended that careful attention be paid to the contents of any data, and that the author be contacted with any questions regarding appropriate use. If you find any errors or omissions, please report them to polardatacentre@bas.ac.uk.

Title

European Petroleum Survey Group (EPSG) Geodetic Parameter Registry

Date (Publication)

2008-11-12

Cited responsible party

Organisation name	Individual name	Electronic mail address	Role
European Petroleum Survey Group		EPSGadministrator@iogp.org	Publisher

Unique resource identifier

urn:ogc:def:crs:EPSG::3031

Version

6.18.3

Hierarchy level

Dataset

Statement

Methodology:

The marine invertebrates were collected during the RV Polarstern expedition PS136.

The pelagic copepods Calanus finmarchicus and Calanus hyperboreus were collected using a BONGO net and a Multi-net.

Benthic and epibenthic marine invertebrates were collected using an epibenthic sledge.

Specimens for lipid and bulks stable isotope analyses were sorted live, photographed and fixed -80 °C in cryovials.

Lipid extractions and Gas chromatography-mass spectrometry (GC-MS) analyses were carried out in the Jeffreys' lab at the University of Liverpool following their standard protocol. Compounds were identified either by comparison of their mass spectra and relative retention indices with those available in the literature, and/or by comparison with authentic standards. Shorthand notations of fatty acids and alcohols follows the IUPAC (International Union of Pure and Applied Chemistry, hhtp://www.iupac.org) systematic nomenclature 'n-x' notation.

Quantitative data were calculated by comparison of peak areas of the internal standard with those of the compounds of interest, using the total ion current (TIC) chromatogram. The relative response factors of the analytes were determined individually for 36 representative fatty acids and sterols using authentic standards. Response factors for analytes where standards were unavailable were assumed to be identical to those of available compounds of the same class.

Bulk stable isotope analyses were carried out in the Jeffreys' lab at the University of Liverpool following their standard protocol. Isotope values were corrected using internationally certified isotopic reference materials USGS40 and USGS41a (Reston Stable Isotope Laboratory) and are reported in delta-notation (per mille) relative to atmospheric nitrogen (N2) and Vienna PeeDee Belemnite carbonate (V-PDB) scales. An internal standard of homogenized prawn (Penaeus vannamei) with well characterized d15N values (6.50 per mille +/- 0.16 per mille) was used to measure precision.

In the data analyses Quantitative lipid constituent data were separated into three datasets which included lipid compound groups (e.g., saturated fatty acids, monounsaturated fatty acids), individual fatty acids, and individual fatty alcohols. These data were converted to relative abundance (mol%) of total identified lipid compounds, fatty acids, or fatty alcohols, respectively. The relative abundance of fatty acid isomers with the same number of carbon molecules and double bounds but unidentified omega groups were summed and labelled as 'X1:X2(iso)'.

Data collection:

The epibenthic sledge used for the collection of the benthic invertebrates was DZMB Senckenberg Wilhelmshaven build following the design by Brenke (2005).

Gas chromatography-mass spectrometry (GC-MS) analyses of the total lipid extracts were conducted using a GC Trace 1300 fitted with a split-splitless injector and column DB-5MS. The eluent from the GC was transferred directly via a transfer line (320 degrees Celsius) to the electron impact source of a Thermoquest ISQMS single quadrupole mass spectrometer. Mass data were collected at a resolution of 600, cycling every second from 50 to 600 Da and were processed using Xcalibur software.

Bulk stable isotope samples were analysed following acid vapour treatment (HCL; 12 h; Yamamuro and Kayanne, 1995), using an ECS8020 elemental analyser (NC Technologies) coupled to a Delta V isotope ratio mass spectrometer (Thermo Scientific).

Data quality:

The identification to species in the calanoid copepods were carried out by experienced zooplankton scientists, while the macrobenthic invertebrates were consistently identified to higher taxonomic levels (Phylum, class, order) but only selected specimens identified to Genus or species.

Lipid and bulk stable isotope data were quality controlled using standards.

Metadata

File identifier

e115d087-e3e4-4794-b4e0-18262b8e6400 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Dataset

Hierarchy level name

dataset

Date stamp

2025-10-08

Metadata standard name

ISO 19115 Geographic Information - Metadata

Metadata standard version

ISO 19115:2003(E)

Metadata author

Organisation name	Individual name	Electronic mail address	Role
NERC EDS UK Polar Data Centre		polardatacentre@bas.ac.uk	Point of contact