BGS Hosted Metadata
Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions
This dataset contains information about the number of bacterial colonies recovered from plastics beads that were incubated in a 96-well plate for 24 hours at 37 degrees Celsius. Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control. The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
Simple
- Date (Publication)
- 2024-10-28
- Citation identifier
- https://catalogue.ceh.ac.uk/id/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
- Citation identifier
- doi: / 10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
- Other citation details
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Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre 10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
- Point of contact
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Organisation name Individual name Electronic mail address Role Brunel University London McCarthy, R.R.
Point of contact Brunel University London Howard, S.
Author Brunel University London McCarthy, R.R.
Author Brunel University London EMAIL NOT PROVIDED
Owner NERC EDS Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian
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GEMET - INSPIRE themes, version 1.0
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Human Health and Safety
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- Keywords
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- Pollution
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Biofilm
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plastic degradation
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polyesterase
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Escherichia coli
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Other constraints
- This resource is licensed under a Creative Commons Attribution 4.0 International Licence
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
- Language
- English
- Character set
- UTF8
- Topic category
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- Health
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/eef3a20c-cb4c-415f-b4be-4d1c31fb6015 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/eef3a20c-cb4c-415f-b4be-4d1c31fb6015.zip Supporting information
- Hierarchy level
- Non geographic dataset
- Other
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nonGeographicDataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted.
Metadata
- File identifier
- eef3a20c-cb4c-415f-b4be-4d1c31fb6015 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Non geographic dataset
- Hierarchy level name
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nonGeographicDataset
- Date stamp
- 2026-02-06T13:36:12
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
