BGS Hosted Metadata
Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022
This dataset holds realistic environmental concentrations of potential pathogens colonising microplastic beads (nurdles) collected from ten Scottish beaches. Observed measures include concentrations and the present genes of Klebsiella bacteria isolated from the nurdles, as well as the salinity, electrical conductivity, turbidity, and polymer composition of the nurdles. In addition, an infection experiment was conducted to measure the virulence of the Klebsiella bacteria. Galleria melonella larvae were infected with the isolated Klebsiella bacteria and the number of living larvae was recorded. Full details about this dataset can be found at https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
Simple
- Date (Publication)
- 2024-08-09
- Citation identifier
- https://catalogue.ceh.ac.uk/id/fdd72810-a57f-444f-83cf-f2351ef4c940
- Citation identifier
- doi: / 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
- Other citation details
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Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
- Point of contact
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Organisation name Individual name Electronic mail address Role University of Stirling Metcalf, R. Author University of Stirling Messer, L.
Author University of Stirling White, H.L.
Author University of Stirling Ormsby, M.
Author University of Stirling Matallana-Surget, S.
Author University of Stirling Quilliam, R. Author University of Stirling Metcalf, R.
Point of contact University of Stirling Owner NERC EDS Environmental Information Data Centre Publisher NERC EDS Environmental Information Data Centre Custodian
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GEMET - INSPIRE themes, version 1.0
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Environmental Monitoring Facilities
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- Keywords
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- Environmental survey
- Pollution
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public health
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plastic pollution
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human pathogens
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plastisphere
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antimicrobial resistance
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virulence
- Access constraints
- Other restrictions
- Other constraints
- no limitations
- Use constraints
- Other restrictions
- Use constraints
- Other restrictions
- Other constraints
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If you reuse this data, you should cite: Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
- Spatial representation type
- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Environment
- Begin date
- 2022-11-01
- End date
- 2022-11-30
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- Unique resource identifier
- OSGB 1936 / British National Grid
- Distribution format
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Name Version Comma-separated values (CSV)
- Distributor contact
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre
Distributor
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/data/fdd72810-a57f-444f-83cf-f2351ef4c940 Download the data
- OnLine resource
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Protocol Linkage Name https://data-package.ceh.ac.uk/sd/fdd72810-a57f-444f-83cf-f2351ef4c940.zip Supporting information
- Hierarchy level
- Dataset
- Other
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dataset
Conformance result
- Title
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Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
- Date (Publication)
- 2010-12-08
- Statement
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Microplastic beads were collected from ten Scottish beaches (Erskine, Yellowcraig, Largs, Irvine, Ayr, Turnberry, Montrose, Broughty Ferry, Portobello and Eyemouth) and bacteria were removed for quantification of target bacteria. Bacteria were quantified using selective media and positive colony-forming units (CFU) were enumerated. Water temperature and salinity at each site were also measured with a thermometer and a salinity refractometer (RGBS). Fourier Transform Infrared Spectrometry (FTIR) was conducted on a Thermo Scientific Nicolet is50 FTIR to identify the polymer composition of the nurdles. The virulence of the isolated Klebsiella was studied for one of the sites, Brought Ferry. Galleria mellonella model of infection was used to determine the virulence of each of the selected Klebsiella strains following their isolation from the microplastic beads and sand. After injection, larvae were incubated at and inspected 24, 48 and 72 h post-injection; larvae were considered dead when they did not respond to touch stimuli.
Metadata
- File identifier
- fdd72810-a57f-444f-83cf-f2351ef4c940 XML
- Metadata language
- English
- Character set
- 8859 Part 1
- Hierarchy level
- Dataset
- Hierarchy level name
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dataset
- Date stamp
- 2025-11-13T16:16:05
- Metadata standard name
- UK GEMINI
- Metadata standard version
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2.3
- Metadata author
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Organisation name Individual name Electronic mail address Role NERC EDS Environmental Information Data Centre Point of contact
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